Investigator, Howard Hughes Medical Institute at UCLA

Tamir Gonen is a membrane biophysicist and an expert in crystallography and cryo-EM. Gonen is a professor of Biological Chemistry and Physiology at the David Geffen School of Medicine of the University of California, Los Angeles, an Investigator of the Howard Hughes Medical Institute, and a Member of the Royal Society of New Zealand. He received a Career Development Award from the American Diabetes Association and was an Early Career Scientist of HHMI. Gonen served on several study sections of the National Institutes of Health and acted as ad hoc reviewer for several international funding agencies. In 2011 while leading a lab at the HHMI Janelia Research Campus he began developing microcrystal electron diffraction (MicroED) as a new method for structural biology. In 2017 Dr Gonen moved his laboratory to the David Geffen School of Medicine of the University of California, Los Angeles as an Investigator of the Howard Hughes Medical Institute and a Professor of Biological Chemistry and Physiology, where he continues studying membrane protein structure and function using cryo-EM and MicroED. With this method Dr Gonen has pushed the boundaries of cryo-EM and determined several previously unknown structures at resolutions better than 1 Å. Gonen authored more than 120 publications and several of his past trainees are now faculty around the world at top universities.

Honors

1996Dean’s list—Organic Chemistry, University of Auckland, New Zealand
1996Dean’s list—Inorganic Chemistry, University of Auckland, New Zealand
1997Center for Gene Technology Research Scholarship, University of Auckland, New Zealand
1997Dean’s List—Inorganic Chemistry, University of Auckland, New Zealand
1998Senior prize in Biological Sciences, University of Auckland, New Zealand
1998First class honors in Biological Sciences, University of Auckland, New Zealand
1999University of Auckland Doctoral Scholarship, University of Auckland, New Zealand
2000Contestable Travel Fund Award, University of Auckland, New Zealand
2001Contestable Travel Fund Award, University of Auckland, New Zealand
2009American Diabetes Association Career Development Award
2009Howard Hughes Medical Institute Early Career Scientist
2010New investigator, Science in Medicine Lecture
2012Member, The Royal Society of New Zealand
2017Investigator, Howard Hughes Medical Institute
2018Chair elect, Biophysical Society cryo-EM subgroup

Publications in PubMed

Publications

131 entries « 2 of 3 »

2017

Liu, Shian; Hattne, Johan; Reyes, Francis E; Sanchez-Martinez, Silvia; de la Cruz, Jason M; Shi, Dan; Gonen, Tamir

Atomic resolution structure determination by the cryo-EM method MicroED Journal Article

In: Protein Sci., vol. 26, no. 1, pp. 8–15, 2017.

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de la Cruz, M. Jason; Martynowycz, Michael; Hattne, Johan; Shi, Dan; Gonen, Tamir

A method to minimize condenser lens-induced hysteresis effects in a JEOL JEM-3200FSC microscope to enable stable cryoEM low-dose operations Online

bioRxiv 2017.

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Krotee, Pascal; Griner, Sarah L; Sawaya, Michael R; Cascio, Duilio; Rodriguez, Jose A; Shi, Dan; Philipp, Stephan; Murray, Kevin; Saelices, Lorena; Lee, Ji; Seidler, Paul; Glabe, Charles G; Jiang, Lin; Gonen, Tamir; Eisenberg, David S

Common fibrillar spines of amyloid-β and human islet amyloid polypeptide revealed by microelectron diffraction and structure-based inhibitors Journal Article

In: J. Biol. Chem., vol. 293, no. 8, pp. 2888–2902, 2017.

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de la Cruz, M. Jason; Hattne, Johan; Shi, Dan; Rodriguez, Jose A.; Reyes, Francis E.; Gonen, Tamir

Micro- and nanocrystal preparation for MicroED and XFEL serial crystallography by fragmentation of imperfect crystals Online

Nature Protocol Exchange 2017.

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de la Cruz, Jason M; Hattne, Johan; Shi, Dan; Seidler, Paul; Rodriguez, Jose; Reyes, Francis E; Sawaya, Michael R; Cascio, Duilio; Weiss, Simon C; Kim, Sun Kyung; Hinck, Cynthia S; Hinck, Andrew P; Calero, Guillermo; Eisenberg, David; Gonen, Tamir

Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED Journal Article

In: Nat. Methods, vol. 14, no. 4, pp. 399–402, 2017.

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Krotee, Pascal; Rodriguez, Jose A; Sawaya, Michael R; Cascio, Duilio; Reyes, Francis E; Shi, Dan; Hattne, Johan; Nannenga, Brent L; Oskarsson, Marie E; Philipp, Stephan; Griner, Sarah; Jiang, Lin; Glabe, Charles G; Westermark, Gunilla T; Gonen, Tamir; Eisenberg, David S

Atomic structures of fibrillar segments of hIAPP suggest tightly mated β-sheets are important for cytotoxicity Journal Article

In: Elife, vol. 6, 2017.

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Gonen, Tamir (Ed.)

Challenges in Structural Biology Conference

HHMI Janelia Research Campus, 2017.

2016

Nannenga, Brent L; Gonen, Tamir

MicroED opens a new era for biological structure determination Journal Article

In: Curr. Opin. Struct. Biol., vol. 40, pp. 128–135, 2016.

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Sawaya, Michael R; Rodriguez, Jose; Cascio, Duilio; Collazo, Michael J; Shi, Dan; Reyes, Francis E; Hattne, Johan; Gonen, Tamir; Eisenberg, David S

Ab initio structure determination from prion nanocrystals at atomic resolution by MicroED Journal Article

In: Proc. Natl. Acad. Sci. U.S.A., vol. 113, no. 40, pp. 11232–11236, 2016.

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Bale, Jacob B; Gonen, Shane; Liu, Yuxi; Sheffler, William; Ellis, Daniel; Thomas, Chantz; Cascio, Duilio; Yeates, Todd O; Gonen, Tamir; King, Neil P; Baker, David

Accurate design of megadalton-scale two-component icosahedral protein complexes Journal Article

In: Science, vol. 353, no. 6297, pp. 389–394, 2016.

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Hsia, Yang; Bale, Jacob B; Gonen, Shane; Shi, Dan; Sheffler, William; Fong, Kimberly K; Nattermann, Una; Xu, Chunfu; Huang, Po‐Ssu.; Ravichandran, Rashmi; Yi, Sue; Davis, Trisha N; Gonen, Tamir; King, Neil P; Baker, David

Design of a hyperstable 60-subunit protein icosahedron Journal Article

In: Nature, vol. 535, no. 7610, pp. 136–139, 2016.

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Hattne, Johan; Shi, Dan; de la Cruz, Jason M; Reyes, Francis E; Gonen, Tamir

Modeling truncated pixel values of faint reflections in MicroED images Journal Article

In: J Appl Crystallogr, vol. 49, no. Pt 3, pp. 1029–1034, 2016.

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Shi, Dan; Nannenga, Brent L; de la Cruz, Jason M; Liu, Jinyang; Sawtelle, Steven; Calero, Guillermo; Reyes, Francis E; Hattne, Johan; Gonen, Tamir

The collection of MicroED data for macromolecular crystallography Journal Article

In: Nat Protoc, vol. 11, no. 5, pp. 895–904, 2016.

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Meyer, Peter A; Socias, Stephanie; Key, Jason; Ransey, Elizabeth; Tjon, Emily C; Buschiazzo, Alejandro; Lei, Ming; Botka, Chris; Withrow, James; Neau, David; Rajashankar, Kanagalaghatta; Anderson, Karen S; Baxter, Richard H; Blacklow, Stephen C; Boggon, Titus J; Bonvin, Alexandre M J J; Borek, Dominika; Brett, Tom J; Caflisch, Amedeo; Chang, Chung-I; Chazin, Walter J; Corbett, Kevin D; Cosgrove, Michael S; Crosson, Sean; Dhe‐Paganon, Sirano; Cera, Enrico Di; Drennan, Catherine L; Eck, Michael J; Eichman, Brandt F; Fan, Qing R; Ferré‐D’Ámaré, Adrian R; Fromme, Christopher J; Garcia, Christopher K; Gaudet, Rachelle; Gong, Peng; Harrison, Stephen C; Heldwein, Ekaterina E; Jia, Zongchao; Keenan, Robert J; Kruse, Andrew C; Kvansakul, Marc; McLellan, Jason S; Modis, Yorgo; Nam, Yunsun; Otwinowski, Zbyszek; Pai, Emil F; Pereira, Pedro José Barbosa; Petosa, Carlo; Raman, C S; Rapoport, Tom A; Roll‐Mecak, Antonina; Rosen, Michael K; Rudenko, Gabby; Schlessinger, Joseph; Schwartz, Thomas U; Shamoo, Yousif; Sondermann, Holger; Tao, Yizhi J; Tolia, Niraj H; Tsodikov, Oleg V; Westover, Kenneth D; Wu, Hao; Foster, Ian; Fraser, James S; Maia, Felipe R N C; Gonen, Tamir; Kirchhausen, Tom; Diederichs, Kay; Crosas, Mercè; Sliz, Piotr

Data publication with the structural biology data grid supports live analysis Journal Article

In: Nat Commun, vol. 7, pp. 10882, 2016.

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Rodriguez, J A; Gonen, T

High-Resolution Macromolecular Structure Determination by MicroED, a cryo-EM Method Book Chapter

In: Methods in Enzymology, vol. 579, Chapter 14, pp. 369–392, 2016.

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2015

Rodriguez, Jose A; Ivanova, Magdalena I; Sawaya, Michael R; Cascio, Duilio; Reyes, Francis E; Shi, Dan; Sangwan, Smriti; Guenther, Elizabeth L; Johnson, Lisa M; Zhang, Meng; Jiang, Lin; Arbing, Mark A; Nannenga, Brent L; Hattne, Johan; Whitelegge, Julian; Brewster, Aaron S; Messerschmidt, Marc; Boutet, Sébastien; Sauter, Nicholas K; Gonen, Tamir; Eisenberg, David S

Structure of the toxic core of α-synuclein from invisible crystals Journal Article

In: Nature, vol. 525, no. 7570, pp. 486–490, 2015.

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Bale, Jacob B; Park, Rachel U; Liu, Yuxi; Gonen, Shane; Gonen, Tamir; Cascio, Duilio; King, Neil P; Yeates, Todd O; Baker, David

Structure of a designed tetrahedral protein assembly variant engineered to have improved soluble expression Journal Article

In: Protein Sci., vol. 24, no. 10, pp. 1695–1701, 2015.

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Hattne, Johan; Reyes, Francis E; Nannenga, Brent L; Shi, Dan; de la Cruz, Jason M; Leslie, Andrew G W; Gonen, Tamir

MicroED data collection and processing Journal Article

In: Acta Crystallogr A Found Adv, vol. 71, no. Pt 4, pp. 353–360, 2015.

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Gonen, Shane; DiMaio, Frank; Gonen, Tamir; Baker, David

Design of ordered two-dimensional arrays mediated by noncovalent protein-protein interfaces Journal Article

In: Science, vol. 348, no. 6241, pp. 1365–1368, 2015.

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2014

Huang, Po-Ssu; Oberdorfer, Gustav; Xu, Chunfu; Pei, Xue Y; Nannenga, Brent L; Rogers, Joseph M; DiMaio, Frank; Gonen, Tamir; Luisi, Ben; Baker, David

High thermodynamic stability of parametrically designed helical bundles Journal Article

In: Science, vol. 346, no. 6208, pp. 481–485, 2014.

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Nannenga, Brent L; Shi, Dan; Hattne, Johan; Reyes, Francis E; Gonen, Tamir

Structure of catalase determined by MicroED Journal Article

In: Elife, vol. 3, pp. e03600, 2014.

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Russell, Alistair B; Wexler, Aaron G; Harding, Brittany N; Whitney, John C; Bohn, Alan J; Goo, Young Ah; Tran, Bao Q; Barry, Natasha A; Zheng, Hongjin; Peterson, Brook S; Chou, Seemay; Gonen, Tamir; Goodlett, David R; Goodman, Andrew L; Mougous, Joseph D

A type VI Secretion-Related Pathway in Bacteroidetes Mediates Interbacterial Antagonism Journal Article

In: Cell Host Microbe, vol. 16, no. 2, pp. 227–236, 2014.

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Wisedchaisri, Goragot; Park, Min-Sun; Iadanza, Matthew G; Zheng, Hongjin; Gonen, Tamir

Proton-coupled sugar transport in the prototypical major facilitator superfamily protein XylE Journal Article

In: Nat Commun, vol. 5, pp. 4521, 2014.

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Nannenga, Brent L; Shi, Dan; Leslie, Andrew G W; Gonen, Tamir

High-resolution structure determination by continuous-rotation data collection in MicroED Journal Article

In: Nat. Methods, vol. 11, no. 9, pp. 927–930, 2014.

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Nannenga, Brent L; Gonen, Tamir

Protein structure determination by MicroED Journal Article

In: Curr. Opin. Struct. Biol., vol. 27, pp. 24–31, 2014.

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Gonen, Tamir; Waksman, Gabriel

Editorial overview: Membranes: recent methods in the study of membrane protein structure Journal Article

In: Curr. Opin. Struct. Biol., vol. 27, pp. iv-v, 2014.

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King, Neil P; Bale, Jacob B; Sheffler, William; McNamara, Dan E; Gonen, Shane; Gonen, Tamir; Yeates, Todd O; Baker, David

Accurate design of co-assembling multi-component protein nanomaterials Journal Article

In: Nature, vol. 510, no. 7503, pp. 103–108, 2014.

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Iadanza, Matthew G; Gonen, Tamir

A suite of software for processing MicroED data of extremely small protein crystals Journal Article

In: J Appl Crystallogr, vol. 47, no. Pt 3, pp. 1140–1145, 2014.

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Anderson, Thomas M.; Clay, Mary C.; Cioffi, Alexander G.; Diaz, Katrina A.; Hisao, Grant S.; Tuttle, Marcus D.; Nieuwkoop, Andrew J.; Comellas, Gemma; Maryum, Nashrah; Wang, Shu; Uno, Brice E.; Wildeman, Erin L.; Gonen, Tamir; Rienstra, Chad M.; Burke, Martin D.

Amphotericin forms an extramembranous and fungicidal sterol sponge Journal Article

In: Nat. Chem. Biol., vol. 10, no. 5, pp. 400–406, 2014.

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2013

Shi, Dan; Nannenga, Brent L; Iadanza, Matthew G; Gonen, Tamir

Three-dimensional electron crystallography of protein microcrystals Journal Article

In: Elife, vol. 2, pp. e01345, 2013.

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Smith, Donelson F; Reichow, Steve L; Esseltine, Jessica L; Shi, Dan; Langeberg, Lorene K; Scott, John D; Gonen, Tamir

Intrinsic disorder within an AKAP-protein kinase A complex guides local substrate phosphorylation Journal Article

In: Elife, vol. 2, pp. e01319, 2013.

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Gold, Matthew G; Gonen, Tamir; Scott, John D

Local cAMP signaling in disease at a glance Journal Article

In: J. Cell. Sci., vol. 126, no. Pt 20, pp. 4537–4543, 2013.

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Reichow, Stephen; Gonen, Tamir

Dynamic Modulation of Water Permeability in the Lens Aquaporin-0 Inproceedings

In: Proceedings of Microscopy & Microanalysis, pp. 50–51, 2013.

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Silverman, Julie M; Agnello, Danielle M; Zheng, Hongjin; Andrews, Benjamin T; Li, Mo; Catalano, Carlos E; Gonen, Tamir; Mougous, Joseph D

Haemolysin Coregulated Protein Is an Exported Receptor and Chaperone of Type VI Secretion Substrates Journal Article

In: Mol. Cell, vol. 51, no. 5, pp. 584–593, 2013.

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Reichow, Steve L; Clemens, Daniel M; Freites, Alfredo J; Nemeth-Cahalan, Karin L; Heyden, Matthias; Tobias, Douglas J; Hall, James E; Gonen, Tamir

Allosteric mechanism of water-channel gating by Ca²⁺-calmodulin Journal Article

In: Nat. Struct. Mol. Biol., vol. 20, no. 9, pp. 1085–1092, 2013.

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Zheng, Hongjin; Wisedchaisri, Goragot; Gonen, Tamir

Crystal structure of a nitrate/nitrite exchanger Journal Article

In: Nature, vol. 497, no. 7451, pp. 647–651, 2013.

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Nannenga, Brent L; Iadanza, Matthew G; Vollmar, Breanna S; Gonen, Tamir

Overview of Electron Crystallography of Membrane Proteins: Crystallization and Screening Strategies Using Negative Stain Electron Microscopy Journal Article

In: Curr Protoc Protein Sci, vol. 72, no. 1, pp. 17.15.1–17.15.11, 2013.

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Shi, Liang; Zheng, Hongjin; Zheng, Hui; Borkowski, Brian A; Shi, Dan; Gonen, Tamir; Jiang, Qiu-Xing

Voltage sensor ring in a native structure of a membrane-embedded potassium channel Journal Article

In: Proc. Natl. Acad. Sci. U.S.A., vol. 110, no. 9, pp. 3369–3374, 2013, (Retracted).

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2012

Filbin, Megan E; Vollmar, Breanna S; Shi, Dan; Gonen, Tamir; Kieft, Jeffrey S

HCV IRES manipulates the ribosome to promote the switch from translation initiation to elongation Journal Article

In: Nat. Struct. Mol. Biol., vol. 20, no. 2, pp. 150–158, 2012.

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Wisedchaisri, Goragot; Gonen, Tamir

Phasing Electron Diffraction Data by Molecular Replacement: Strategy for Structure Determination and Refinement Book Chapter

In: Electron Crystallography of Soluble and Membrane Proteins, vol. 955, Chapter 14, pp. 243–272, 2012.

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Gonen, Tamir

The Collection of High-Resolution Electron Diffraction Data Book Chapter

In: Electron Crystallography of Soluble and Membrane Proteins, vol. 955, Chapter 9, pp. 153–169, 2012.

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Stokes, David L; Ubarretxena-Belandia, Iban; Gonen, Tamir; Engel, Andreas

High-Throughput Methods for Electron Crystallography Book Chapter

In: Electron Crystallography of Soluble and Membrane Proteins, vol. 955, Chapter 15, pp. 273–296, 2012.

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Umbreit, Neil T.; Gestaut, Daniel R.; Tien, Jerry F.; Vollmar, Breanna S.; Gonen, Tamir; Asbury, Charles L.; Davis, Trisha N.

The Ndc80 kinetochore complex directly modulates microtubule dynamics Journal Article

In: Proc. Natl. Acad. Sci. U.S.A., vol. 109, no. 40, pp. 16113–16118, 2012.

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Gonen, Shane; Akiyoshi, Bungo; Iadanza, Matthew G; Shi, Dan; Duggan, Nicole; Biggins, Sue; Gonen, Tamir

The structure of purified kinetochores reveals multiple microtubule-attachment sites Journal Article

In: Nat. Struct. Mol. Biol., vol. 19, no. 9, pp. 925–929, 2012.

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Gonen, Tamir; Waksman, Gabriel

Recent progress in membrane protein structures and investigation methods Journal Article

In: Curr. Opin. Struct. Biol., vol. 22, no. 4, pp. 467–468, 2012.

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Jiang, Qiu-Xing; Gonen, Tamir

The influence of lipids on voltage-gated ion channels Journal Article

In: Curr. Opin. Struct. Biol., vol. 22, no. 4, pp. 529–536, 2012.

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King, Neil P; Sheffler, William; Sawaya, Michael R; Vollmar, Breanna S; Sumida, John P; André, Ingemar; Gonen, Tamir; Yeates, Todd O; Baker, David

Computational Design of Self-Assembling Protein Nanomaterials with Atomic Level Accuracy Journal Article

In: Science, vol. 336, no. 6085, pp. 1171–1174, 2012.

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2011

Lo, Sheng-Ying; Brett, Christopher L; Plemel, Rachael L; Vignali, Marissa; Fields, Stanley; Gonen, Tamir; Merz, Alexey J

Intrinsic tethering activity of endosomal Rab proteins Journal Article

In: Nat. Struct. Mol. Biol., vol. 19, no. 1, pp. 40–47, 2011.

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Gold, Matthew G; Reichow, Steve L; O'Neill, Susan E; Weisbrod, Chad R; Langeberg, Lorene K; Bruce, James E; Gonen, Tamir; Scott, John D

AKAP2 anchors PKA with aquaporin-0 to support ocular lens transparency Journal Article

In: EMBO Mol Med, vol. 4, no. 1, pp. 15–26, 2011.

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Wisedchaisri, Goragot; Reichow, Steve L; Gonen, Tamir

Advances in Structural and Functional Analysis of Membrane Proteins by Electron Crystallography Journal Article

In: Structure, vol. 19, no. 10, pp. 1381–1393, 2011.

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131 entries « 2 of 3 »