The Gonen laboratory will hold the 7th MicroED course in 2022. The course will be held in person December 11–14, 2022 at UCLA. The course is organized by Tamir Gonen (UCLA) and Brent Nannenga (ASU).

Topics covered

  • Sample preparation methodologies for proteins and small molecules
  • MicroED data collection
  • Camera performance
  • Cryo-FIB-milling procedures for MicroED
  • Data analysis software, structure solution, and refinement

Tentative schedule

TimeTitle
Sunday Dec. 11th 2022
5:00 PMWelcome Dinner
Monday Dec. 12th 2022
8:30 AM – 9:00 AMBreakfast
9:00 AM – 9:20 AMIntroduction to MicroED and welcoming notes
9:20 AM – 9:40 AMSample preparation
9:40 AM – 10:00 AMFIB milling for MicroED
10:00 AM – 10:30 AMBreak
10:30 AM – 11:00 AMMicroED data collection with EPUD
11:00 AM – 12:00 PMInstallation and testing of software for workshop
12:00 PM – 1:00 PMLunch
1:00 PM – 5:00 PMPractical sessions I
5:00 PMDinner
Tuesday Dec. 13th 2022
8:30 AM – 9:00 AMBreakfast
9:00 AM – 10:00 AMArchiving, structure deposition, and validation
10:00 AM – 10:30 AMCameras for MicroED
10:30 AM – 11:00 AMBreak
11:00 AM – 11:30 AMEquipment for MicroED
11:30 AM – 12:00 PMChemical crystallography with MicroED
12:00 PM – 1:00 PMLunch
1:00 PM – 5:00 PMPractical sessions II
5:00 PMDinner
Wednesday Dec. 14th 2022
8:30 AM – 9:00 AMBreakfast
9:00 AM – 9:30 AMAutomated data collection for MicroED
9:30 AM – 10:00 AMData processing and structure determination of small molecules
10:00 AM – 10:30 AMMicroED with GPCRs
10:30 AM – 11:00 AMEnzyme structure determination by MicroED
11:00 AM – 12:00 PMOpen discussion
12:00 PM – 1:00 PMLunch
1:00 PM – 5:00 PMPractical sessions III (Sample preparation, data collection and processing)
5:00 PMDinner and closing

Organizers

MicroED Imaging Center (MEDIC) at UCLA

Instructors

Tamir Gonen (UCLA), Brent Nannenga (ASU), Johan Hattne (UCLA), Michael Martynowycz (UCLA), Max Clabbers (UCLA), Callie Saeher (UCLA), Emma Danelius (UCLA), and Anna Shiriaeva (UCLA).

Cost

The cost for academic attendees is $500. The cost for attendees outside academia is $1,500. The cost of the workshop covers meals and other expenses associated with the course.

Application

This course is not accepting new applications.