Laboratory of molecular electron microscopy
The laboratory of molecular electron microscopy, headed by Tamir Gonen, is located at the David Geffen Medical School of Medicine, University of California, Los Angeles.
Our laboratory studies the structures of membrane proteins. Based on structure we try to understand function and what goes wrong in disease. We focus primarily on proteins in the blood-brain barrier. The long-standing question in our laboratory is how the thousands of membrane channels and transporters that exist in the cell membrane work together to help cells maintain homeostasis. With that question in mind, we study membrane proteins that are involved in nutrient, ion, and water uptake, waste removal, signaling, and communication.
Our laboratory is multidisciplinary. Over the last decade we have employed structural biology techniques such as electron cryo-microscopy (cryo-EM), X-ray crystallography, NMR, molecular dynamics simulations, and used membrane biochemistry and biophysics to understand the function of the proteins of interest. Within electron microscopy we have published papers using electron tomography, single particle reconstructions, and electron crystallography. However, our specialty lies in electron diffraction.
Part of our laboratory is also devoted to method development in cryo-EM. In recent years we have developed two important methods in electron diffraction, namely fragment-based phase extension and MicroED.
- Callie Saeher awarded a Ruth L. Kirschstein fellowshipCallie Saeher is awarded a postdoctoral national research service award fellowship from the National Institutes of Health for her work on mTORC1 activation. The purpose of the fellowship is to train promising candidates to become productive, independent investigators in scientific health-related research fields.
- Emma Danelius and Xuelang Mu to deliver keynote talks at ACA 2023Emma Danelius and Xuelang Mu have been selected to deliver keynote talks in the transactions symposium at this year’s annual meeting of the American Crystallographic Association. The meeting will be held in Baltimore, MD in July 2023.
- Cody Gillman wins Etter awardCody Gillman was awarded the Margaret C. Etter Student Lecturer Award by the American Crystallographic Association for his work on the Design and Implementation of Suspended Drop Crystallization.
- Cody Gillman awarded an Audree Fowler fellowshipCody Gillman is one of five 2022 Audree Fowler fellows. The fellowship was awarded for his excellent work on determining the structure of a toxin‐antibody complex by MicroED.
- Michael Martynowycz receives the Sidhu AwardMichael Martynowycz is the recipient of the 2022 Sidhu Award from the Pittsburgh Diffraction Society for the scientific impact of his work on methods development and his dedication to advancing the field of crystallography through MicroED. The award, in memory of Professor Surhain S. Sidhu, honors significant contributions to the science of crystallography and/or diffraction … Read more
- A. L. Patterson AwardTamir Gonen is awarded the 2023 A. L. Patterson Award from the ACA: The Structural Science Society for his pioneering work on the cryo-EM method MicroED. RefleXions published an essay on two decades of MicroED development.
- Hermina Wieske awarded three travel scholarshipsHermina Wieske is awarded travel scholarships from Liljewalchs stipendiestiftelse, G. Bergmarks stiftelse, and Stiftelsen Bengt Lundqvists minne to visit the Gonen laboratory.
- Cody Gillman obtains NIH T32 fellowshipCody Gillman was awarded an NIH T32 fellowship in Muscle Cell Biology, Pathophysiology and Therapeutics at UCLA for his research on using MicroED to analyze membrane proteins in lipids.
- Emma Danelius wins the 2022 Suzanne Eaton Memorial PrizeThe prize is intended to recognize a scientist who displays the traits characterized by Dr. Suzanne Eaton, PhD: excellence in their work, passion for their discipline, and a caring personality for their colleagues.