Investigator, Howard Hughes Medical Institute at UCLA

Tamir Gonen is a membrane biophysicist and an expert in crystallography and cryo-EM. Gonen is a professor of Biological Chemistry and Physiology at the David Geffen School of Medicine of the University of California, Los Angeles, an Investigator of the Howard Hughes Medical Institute, and a Member of the Royal Society of New Zealand. He received a Career Development Award from the American Diabetes Association and was an Early Career Scientist of HHMI. Gonen served on several study sections of the National Institutes of Health and acted as ad hoc reviewer for several international funding agencies. In 2011 while leading a lab at the HHMI Janelia Research Campus he began developing microcrystal electron diffraction (MicroED) as a new method for structural biology. In 2017 Dr Gonen moved his laboratory to the David Geffen School of Medicine of the University of California, Los Angeles as an Investigator of the Howard Hughes Medical Institute and a Professor of Biological Chemistry and Physiology, where he continues studying membrane protein structure and function using cryo-EM and MicroED. With this method Dr Gonen has pushed the boundaries of cryo-EM and determined several previously unknown structures at resolutions better than 1 Å. Gonen authored more than 120 publications and several of his past trainees are now faculty around the world at top universities.

Honors

1996Dean’s list—Organic Chemistry, University of Auckland, New Zealand
1996Dean’s list—Inorganic Chemistry, University of Auckland, New Zealand
1997Center for Gene Technology Research Scholarship, University of Auckland, New Zealand
1997Dean’s List—Inorganic Chemistry, University of Auckland, New Zealand
1998Senior prize in Biological Sciences, University of Auckland, New Zealand
1998First class honors in Biological Sciences, University of Auckland, New Zealand
1999University of Auckland Doctoral Scholarship, University of Auckland, New Zealand
2000Contestable Travel Fund Award, University of Auckland, New Zealand
2001Contestable Travel Fund Award, University of Auckland, New Zealand
2009American Diabetes Association Career Development Award
2009Howard Hughes Medical Institute Early Career Scientist
2010New investigator, Science in Medicine Lecture
2012Member, The Royal Society of New Zealand
2017Investigator, Howard Hughes Medical Institute
2018Chair elect, Biophysical Society cryo-EM subgroup
2023A. L. Patterson award, ACA: The Structural Science Society

Publications in PubMed

Publications

137 entries « 1 of 3 »

2022

Clabbers, Max T. B.; Martynowycz, Michael W.; Hattne, Johan; Gonen, Tamir

Hydrogens and hydrogen-bond networks in macromolecular MicroED data Journal Article

In: J Struct Biol X, vol. 6, pp. 100078, 2022.

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Clabbers, Max T. B.; Martynowycz, Michael W.; Hattne, Johan; Nannenga, Brent L.; Gonen, Tamir

Electron-counting MicroED data with the K2 and K3 direct electron detectors Journal Article

In: J Struct Biol, 2022.

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Martynowycz, Michael W.; Shiriaeva, Anna; Clabbers, Max T. B.; Nicolas, William J.; Weaver, Sara J.; Hattne, Johan; Gonen, Tamir

A robust approach for MicroED sample preparation of lipidic cubic phase embedded membrane protein crystals Online

bioRxiv 2022, visited: 26.07.2022.

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Martynowycz, Michael W.; Gonen, Tamir

Unlocking the potential of microcrystal electron diffraction Journal Article

In: Physics Today, vol. 75, iss. 6, pp. 38–42, 2022.

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Nguyen, Chi; Lei, Hsiang-Ting; Lai, Louis Tung Faat; Gallenito, Marc J.; Matthies, Doreen; Gonen, Tamir

Lipid flipping in the omega-3 fatty-acid transporter Online

bioRxiv 2022, visited: 01.06.2022.

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Martynowycz, Michael W; Clabbers, Max T B; Hattne, Johan; Gonen, Tamir

Ab initio phasing macromolecular structures using electron-counted MicroED data Journal Article

In: Nat Methods, vol. 19, iss. 6, pp. 724–729, 2022, ISSN: 1548-7105.

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Porter, Nicholas J; Danelius, Emma; Gonen, Tamir; Arnold, Frances H

Biocatalytic Carbene Transfer Using Diazirines Journal Article

In: J Am Chem Soc, 2022, ISSN: 1520-5126.

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Clabbers, Max T B; Shiriaeva, Anna; Gonen, Tamir

MicroED: conception, practice and future opportunities Journal Article

In: IUCrJ, vol. 9, no. Pt 2, pp. 169–179, 2022, ISSN: 2052-2525.

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Gallenito, Marc J; Gonen, Tamir

Studying membrane proteins with MicroED Journal Article

In: Biochem Soc Trans, vol. 50, no. 1, pp. 231–239, 2022, ISSN: 1470-8752.

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2021

Martynowycz, Michael W; Clabbers, Max T B; Unge, Johan; Hattne, Johan; Gonen, Tamir

Benchmarking the ideal sample thickness in cryo-EM Journal Article

In: Proc Natl Acad Sci U S A, vol. 118, no. 49, 2021, ISSN: 1091-6490.

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Clark, Lisa J.; Bu, Guanhong; Nannenga, Brent L.; Gonen, Tamir

MicroED for the study of protein–ligand interactions and the potential for drug discovery Journal Article

In: Nat Rev Chem, vol. 5, pp. 853–858, 2021.

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Martynowycz, Michael W; Gonen, Tamir

Protocol for the use of focused ion-beam milling to prepare crystalline lamellae for microcrystal electron diffraction (MicroED) Journal Article

In: STAR Protoc, vol. 2, no. 3, pp. 100686, 2021, ISSN: 2666-1667.

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Martynowycz, Michael W.; Shiriaeva, Anna; Ge, Xuanrui; Hattne, Johan; Nannenga, Brent L.; Cherezov, Vadim; Gonen, Tamir

MicroED structure of the human adenosine receptor determined from a single nanocrystal in LCP Journal Article

In: Proc Natl Acad Sci U S A, vol. 118, no. 36, pp. e2106041118, 2021.

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Mu, Xuelang; Gillman, Cody; Nguyen, Chi; Gonen, Tamir

An Overview of Microcrystal Electron Diffraction (MicroED) Journal Article

In: Annu Rev Biochem, vol. 90, pp. 431–450, 2021, ISSN: 1545-4509.

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Lei, Hsiang-Ting; Mu, Xuelang; Hattne, Johan; Gonen, Tamir

A conformational change in the N Terminus of SLC38A9 signals mTORC1 activation Journal Article

In: Structure, vol. 29, no. 5, pp. 426–432.e8, 2021.

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Danelius, E; Gonen, T

Protein and Small Molecule Structure Determination by the Cryo-EM Method MicroED Book Chapter

In: Owens, Raymond J. (Ed.): vol. 2305, pp. 323–342, 2021.

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Martynowycz, M W; Gonen, T

Studying Membrane Protein Structures by MicroED Book Chapter

In: Schmidt-Krey, Ingeborg; Gumbart, James C. (Ed.): vol. 2302, pp. 137–151, 2021.

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Martynowycz, M W; Gonen, T

Microcrystal Electron Diffraction of Small Molecules Journal Article

In: J Vis Exp, no. 169, 2021.

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Danelius, E; Halaby, S; van der Donk, W A; Gonen, T

MicroED in natural product and small molecule research Journal Article

In: Nat Prod Rep, vol. 38, no. 3, 2021.

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Halaby, Steve; Martynowycz, Michael W.; Zhu, Ziyue; Tretiak, Sergei; Zhugayevych, Andriy; Gonen, Tamir; and Martin Seifrid,

Microcrystal Electron Diffraction for Molecular Design of Functional Non-Fullerene Acceptor Structures Journal Article

In: Chem. Mater., vol. 33, no. 3, pp. 966–977, 2021.

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Martynowycz, Michael W.; Gonen, Tamir

Ligand Incorporation into Protein Microcrystals for MicroED by On-Grid Soaking Journal Article

In: Structure, vol. 29, no. 1, pp. 88–95.e2, 2021.

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Gonen, Tamir; Nannenga, Brent (Ed.)

cryoEM: Methods and Protocols Book

Springer US, 2021, ISBN: 978-1-0716-0965-1.

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2020

Martynowycz, M W; Khan, F; Hattne, J; Abramson, J; Gonen, T

MicroED structure of lipid-embedded mammalian mitochondrial voltage-dependent anion channel Journal Article

In: Proc Natl Acad Sci U S A, vol. 117, no. 51, pp. 32380–32385, 2020.

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Zhu, Lan; Bu, Guanhong; Jing, Liang; Shi, Dan; Lee, Ming-Yue; Gonen, Tamir; Liu, Wei; Nannenga, Brent L.

Structure Determination from Lipidic Cubic Phase Embedded Microcrystals by MicroED Journal Article

In: Structure, vol. 28, no. 10, pp. 1149–1159.e4, 2020.

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Nannenga, Brent L.; Gonen, Tamir

Microcrystal electron diffraction methodology and applications Journal Article

In: MRS Bulletin, vol. 44, pp. 956–960, 2020.

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Richards, Logan S.; Millán, Claudia; Miao, Jennifer; Martynowycz, Michael W.; Sawaya, Michael R.; Gonen, Tamir; Borges, Rafael J.; Usón, Isabel; Rodriguez, Jose A.

Fragment-based determination of a proteinase K structure from MicroED data using ARCIMBOLDO_SHREDDER Journal Article

In: Acta Crystallogr D Biol Crystallogr., vol. 76, no. 8, pp. 703-712, 2020.

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Nguyen, Chi; Gonen, Tamir

Beyond protein structure determination with MicroED Journal Article

In: Curr. Opin. Struct. Biol., vol. 64, pp. 51–58, 2020.

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Wolff, A M; Young, I D; Sierra, R G; Brewster, A S; Martynowycz, M W; Nango, E; Sugahara, M; Nakane, T; Ito, K; Aquila, A; Bhowmick, A; Biel, J T; Carbajo, S; Cohen, A E; Cortez, S; Gonzalez, A; Hino, T; Im, D; Koralek, J D; Kubo, M; Lazarou, T S; Nomura, T; Owada, S; Samelson, A J; Tanaka, T; Tanaka, R; Thompson, E M; van den Bedem, H; Woldeyes, R A; Yumoto, F; Zhao, W; Tono, K; Boutet, S; Iwata, S; Gonen, T; Sauter, N K; Fraser, J S; Thompson, M C

Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals Journal Article

In: IUCrJ, vol. 7, no. Pt 2, pp. 306–323, 2020.

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Martynowycz, M W; Hattne, J; Gonen, T

Experimental Phasing of MicroED Data Using Radiation Damage Journal Article

In: Structure, vol. 28, no. 4, pp. 458–464, 2020.

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2019

Dick, Markus; Sarai, Nicholas S; Martynowycz, Michael W; Gonen, Tamir; Arnold, Frances H

Tailoring Tryptophan Synthase TrpB for Selective Quaternary Carbon Bond Formation Journal Article

In: J. Am. Chem. Soc., 2019.

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Martynowycz, Michael W; Zhao, Wei; Hattne, Johan; Jensen, Grant J; Gonen, Tamir

Qualitative Analyses of Polishing and Precoating FIB Milled Crystals for MicroED Journal Article

In: Structure, vol. 27, no. 10, pp. 1594–1600, 2019.

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Hattne, Johan; Martynowycz, Michael W; Penczek, Pawel A; Gonen, Tamir

MicroED with the Falcon III direct electron detector Journal Article

In: IUCrJ, vol. 6, no. Pt 5, pp. 921–926, 2019.

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Warmack, Rebeccah A; Boyer, David R; Zee, Chih-Te; Richards, Logan S; Sawaya, Michael R; Cascio, Duilio; Gonen, Tamir; Eisenberg, David S; Clarke, Steven G

Structure of amyloid-β (20-34) with Alzheimer's-associated isomerization at Asp23 reveals a distinct protofilament interface Journal Article

In: Nat Commun, vol. 10, no. 1, pp. 3357, 2019.

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Ting, Chi P; Funk, Michael A; Halaby, Steve L; Zhang, Zhengan; Gonen, Tamir; van der Donk, Wilfred A

Use of a scaffold peptide in the biosynthesis of amino acid-derived natural products Journal Article

In: Science, vol. 365, no. 6450, pp. 280–284, 2019.

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Martynowycz, Michael W.; Gonen, Tamir

MicroED Structure of Hexagonal Ice Ih Online

ChemRxiv 2019.

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de la Cruz, Jason M; Martynowycz, Michael W; Hattne, Johan; Gonen, Tamir

MicroED data collection with SerialEM Journal Article

In: Ultramicroscopy, vol. 201, pp. 77–80, 2019.

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Nannenga, Brent L; Gonen, Tamir

The cryo-EM method microcrystal electron diffraction (MicroED) Journal Article

In: Nat. Methods, vol. 16, no. 5, pp. 369–379, 2019.

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Martynowycz, Michael W; Zhao, Wei; Hattne, Johan; Jensen, Grant J; Gonen, Tamir

Collection of Continuous Rotation MicroED Data from Ion Beam-Milled Crystals of Any Size Journal Article

In: Structure, vol. 27, no. 3, pp. 545–548, 2019.

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Zee, Chih-Te; Glynn, Calina; Gallagher-Jones, Marcus; Miao, Jennifer; Santiago, Carlos G; Cascio, Duilio; Gonen, Tamir; Sawaya, Michael R; Rodriguez, Jose A

Homochiral and racemic MicroED structures of a peptide repeat from the ice-nucleation protein InaZ Journal Article

In: IUCrJ, vol. 6, no. Pt 2, pp. 197–205, 2019.

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Ma, Jinming; Lei, Hsiang-Ting; Reyes, Francis E; Sanchez-Martinez, Silvia; Sarhan, Maen F; Hattne, Johan; Gonen, Tamir

Structural basis for substrate binding and specificity of a sodium-alanine symporter AgcS Journal Article

In: Proc. Natl. Acad. Sci. U.S.A., vol. 116, no. 6, pp. 2086–2090, 2019.

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Griner, Sarah L; Seidler, Paul; Bowler, Jeannette; Murray, Kevin A; Yang, Tianxiao Peter; Sahay, Shruti; Sawaya, Michael R; Cascio, Duilio; Rodriguez, Jose A; Philipp, Stephan; Sosna, Justyna; Glabe, Charles G; Gonen, Tamir; Eisenberg, David S

Structure-based inhibitors of amyloid beta core suggest a common interface with tau Journal Article

In: Elife, vol. 8, 2019.

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2018

Purdy, Michael D; Shi, Dan; Chrustowicz, Jakub; Hattne, Johan; Gonen, Tamir; Yeager, Mark

MicroED structures of HIV-1 Gag CTD-SP1 reveal binding interactions with the maturation inhibitor bevirimat Journal Article

In: Proc. Natl. Acad. Sci. U.S.A., vol. 115, no. 52, pp. 13258–13263, 2018.

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Jones, Christopher G; Martynowycz, Michael W; Hattne, Johan; Fulton, Tyler J; Stoltz, Brian M; Rodriguez, Jose A; Nelson, Hosea M; Gonen, Tamir

The CryoEM Method MicroED as a Powerful Tool for Small Molecule Structure Determination Journal Article

In: ACS Cent Sci, vol. 4, no. 11, pp. 1587–1592, 2018.

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Lei, Hsiang-Ting; Ma, Jinming; Martinez, Silvia Sanchez; Gonen, Tamir

Crystal structure of arginine-bound lysosomal transporter SLC38A9 in the cytosol-open state Journal Article

In: Nat. Struct. Mol. Biol., vol. 25, no. 6, pp. 522–527, 2018.

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Liu, Shian; Gonen, Tamir

MicroED structure of the NaK ion channel reveals a Na⁺ partition process into the selectivity filter Journal Article

In: Commun Biol, vol. 1, pp. 38, 2018.

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Hattne, Johan; Shi, Dan; Glynn, Calina; Zee, Chih-Te; Gallagher-Jones, Marcus; Martynowycz, Michael W; Rodriguez, Jose A; Gonen, Tamir

Analysis of Global and Site-Specific Radiation Damage in Cryo-EM Journal Article

In: Structure, vol. 26, no. 5, pp. 759–766, 2018.

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Liu, Yuxi; Gonen, Shane; Gonen, Tamir; Yeates, Todd O

Near-atomic cryo-EM imaging of a small protein displayed on a designed scaffolding system Journal Article

In: Proc. Natl. Acad. Sci. U.S.A., vol. 115, no. 13, pp. 3362–3367, 2018.

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Guenther, Elizabeth L; Ge, Peng; Trinh, Hamilton; Sawaya, Michael R; Cascio, Duilio; Boyer, David R; Gonen, Tamir; Zhou, Hong Z; Eisenberg, David S

Atomic-level evidence for packing and positional amyloid polymorphism by segment from TDP-43 RRM2 Journal Article

In: Nat. Struct. Mol. Biol., vol. 25, no. 4, pp. 311–319, 2018.

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Martynowycz, Michael W; Gonen, Tamir

From electron crystallography of 2D crystals to MicroED of 3D crystals Journal Article

In: Curr Opin Colloid Interface Sci, vol. 34, pp. 9–16, 2018.

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Hughes, Michael P; Sawaya, Michael R; Boyer, David R; Goldschmidt, Lukasz; Rodriguez, Jose A; Cascio, Duilio; Chong, Lisa; Gonen, Tamir; Eisenberg, David S

Atomic structures of low-complexity protein segments reveal kinked β sheets that assemble networks Journal Article

In: Science, vol. 359, no. 6376, pp. 698–701, 2018.

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137 entries « 1 of 3 »