CV for Tamir Gonen, Ph.D., MRSNZ

Investigator, Howard Hughes Medical Institute at UCLA

2005–2011Assistant Professor of Biochemistry, University of Washington, Seattle
2009–2011Howard Hughes Medical Institute Early Career Scientist
2011Associate Professor of Biochemistry (Tenured), University of Washington
2011–presentAffiliate Professor of Biochemistry, University of Washington, Seattle, WA
2011–2017Group Leader, HHMI, Janelia Research Campus, Ashburn VA
2017–PresentInvestigator, Howard Hughes Medical Institute.
2017–presentProfessor of Biological Chemistry and Physiology, David Geffen Medical School. UCLA, CA.

Honors

1996Deans List – Organic Chemistry, University of Auckland, New Zealand
1996Deans List – Inorganic Chemistry, University of Auckland, New Zealand
1997Center for Gene Technology Research Scholarship, University of Auckland, New Zealand
1997Deans List – Inorganic Chemistry, University of Auckland, New Zealand
1998Senior Prize in Biological Sciences, University of Auckland, New Zealand
1998First Class Honors in Biological Sciences, University of Auckland, New Zealand
1999University of Auckland Doctoral Scholarship, University of Auckland, New Zealand
2000Contestable Travel Fund Award, University of Auckland, New Zealand
2001Contestable Travel Fund Award, University of Auckland, New Zealand
2009American Diabetes Association Career Development Award
2009Howard Hughes Medical Institute Early Career Scientist
2010New Investigator Science in Medicine Lecture
2012Member, The Royal Society of New Zealand
2017Investigator, Howard Hughes Medical Institute
2018Chair elect, Biophysical Society cryoEM subgroup

Publications in Pubmed

Publications

111 entries « 1 of 3 »

2019

Martynowycz, Michael W; Zhao, Wei; Hattne, Johan; Jensen, Grant J; Gonen, Tamir

Qualitative Analyses of Polishing and Precoating FIB Milled Crystals for MicroED Journal Article

Structure, 27 (10), pp. 1594–1600, 2019.

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Hattne, Johan; Martynowycz, Michael W; Penczek, Pawel A; Gonen, Tamir

MicroED with the Falcon III direct electron detector Journal Article

IUCrJ, 6 (Pt 5), pp. 921–926, 2019.

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Zhu, Lan ; Bu, Guanhong ; Shi, Dan ; Gonen, Tamir ; Liu Wei, Nannenga Brent L

Structure determination from lipidic cubic phase embedded microcrystals by MicroED Online

bioRxiv 2019.

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Warmack, Rebeccah A; Boyer, David R; Zee, Chih-Te; Richards, Logan S; Sawaya, Michael R; Cascio, Duilio; Gonen, Tamir; Eisenberg, David S; Clarke, Steven G

Structure of amyloid-β (20-34) with Alzheimer's-associated isomerization at Asp23 reveals a distinct protofilament interface Journal Article

Nat Commun, 10 (1), pp. 3357, 2019.

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Ting, Chi P; Funk, Michael A; Halaby, Steve L; Zhang, Zhengan; Gonen, Tamir; van der Donk, Wilfred A

Use of a scaffold peptide in the biosynthesis of amino acid-derived natural products Journal Article

Science, 365 (6450), pp. 280–284, 2019.

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Martynowycz, Michael W; Gonen, Tamir

MicroED Structure of Hexagonal Ice Ih Online

ChemRxiv 2019.

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de la Cruz, Jason M; Martynowycz, Michael W; Hattne, Johan; Gonen, Tamir

MicroED data collection with SerialEM Journal Article

Ultramicroscopy, 201 , pp. 77–80, 2019.

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Nannenga, Brent L; Gonen, Tamir

The cryo-EM method microcrystal electron diffraction (MicroED) Journal Article

Nat. Methods, 16 (5), pp. 369–379, 2019.

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Martynowycz, Michael W; Zhao, Wei; Hattne, Johan; Jensen, Grant J; Gonen, Tamir

Collection of Continuous Rotation MicroED Data from Ion Beam-Milled Crystals of Any Size Journal Article

Structure, 27 (3), pp. 545–548, 2019.

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Zee, Chih-Te; Glynn, Calina; Gallagher-Jones, Marcus; Miao, Jennifer; Santiago, Carlos G; Cascio, Duilio; Gonen, Tamir; Sawaya, Michael R; Rodriguez, Jose A

Homochiral and racemic MicroED structures of a peptide repeat from the ice-nucleation protein InaZ Journal Article

IUCrJ, 6 (Pt 2), pp. 197–205, 2019.

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Ma, Jinming; Lei, Hsiang-Ting; Reyes, Francis E; Sanchez-Martinez, Silvia; Sarhan, Maen F; Hattne, Johan; Gonen, Tamir

Structural basis for substrate binding and specificity of a sodium-alanine symporter AgcS Journal Article

Proc. Natl. Acad. Sci. U.S.A., 116 (6), pp. 2086–2090, 2019.

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2018

Purdy, Michael D; Shi, Dan; Chrustowicz, Jakub; Hattne, Johan; Gonen, Tamir; Yeager, Mark

MicroED structures of HIV-1 Gag CTD-SP1 reveal binding interactions with the maturation inhibitor bevirimat Journal Article

Proc. Natl. Acad. Sci. U.S.A., 115 (52), pp. 13258–13263, 2018.

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Jones, Christopher G; Martynowycz, Michael W; Hattne, Johan; Fulton, Tyler J; Stoltz, Brian M; Rodriguez, Jose A; Nelson, Hosea M; Gonen, Tamir

The CryoEM Method MicroED as a Powerful Tool for Small Molecule Structure Determination Journal Article

ACS Cent Sci, 4 (11), pp. 1587–1592, 2018.

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Martynowycz, Michael W; Zhao, Wei ; Hattne, Johen ; Jensen, Grant J; Gonen, Tamir

Collection of continuous rotation MicroED Data from Ion Beam Milled Crystals of Any Size Online

bioRxiv 2018.

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Ma, Jinming ; Lei, Hsiang-Ting ; Gonen, Tamir

A conformational change in the N terminus of SLC38A9 signals mTORC1 activation Online

bioRxiv 2018.

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Lei, Hsiang-Ting; Ma, Jinming; Martinez, Silvia Sanchez; Gonen, Tamir

Crystal structure of arginine-bound lysosomal transporter SLC38A9 in the cytosol-open state Journal Article

Nat. Struct. Mol. Biol., 25 (6), pp. 522–527, 2018.

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Liu, Shian; Gonen, Tamir

MicroED structure of the NaK ion channel reveals a Na⁺ partition process into the selectivity filter Journal Article

Commun Biol, 1 , pp. 38, 2018.

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Hattne, Johan; Shi, Dan; Glynn, Calina; Zee, Chih-Te; Gallagher-Jones, Marcus; Martynowycz, Michael W; Rodriguez, Jose A; Gonen, Tamir

Analysis of Global and Site-Specific Radiation Damage in Cryo-EM Journal Article

Structure, 26 (5), pp. 759–766, 2018.

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Ma, Jinming ; Lei, Hsiang-Ting ; Reyes, Francis E; Sanchez-Martinez, Silvia ; Sarhan, Maen ; Gonen, Tamir

Structural basis for substrate binding and specificity of a sodium/alanine symporter AgcS Online

bioRxiv 2018.

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Liu, Yuxi; Gonen, Shane; Gonen, Tamir; Yeates, Todd O

Near-atomic cryo-EM imaging of a small protein displayed on a designed scaffolding system Journal Article

Proc. Natl. Acad. Sci. U.S.A., 115 (13), pp. 3362–3367, 2018.

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Guenther, Elizabeth L; Ge, Peng; Trinh, Hamilton; Sawaya, Michael R; Cascio, Duilio; Boyer, David R; Gonen, Tamir; Zhou, Hong Z; Eisenberg, David S

Atomic-level evidence for packing and positional amyloid polymorphism by segment from TDP-43 RRM2 Journal Article

Nat. Struct. Mol. Biol., 25 (4), pp. 311–319, 2018.

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Martynowycz, Michael W; Gonen, Tamir

From electron crystallography of 2D crystals to MicroED of 3D crystals Journal Article

Curr Opin Colloid Interface Sci, 34 , pp. 9–16, 2018.

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Hughes, Michael P; Sawaya, Michael R; Boyer, David R; Goldschmidt, Lukasz; Rodriguez, Jose A; Cascio, Duilio; Chong, Lisa; Gonen, Tamir; Eisenberg, David S

Atomic structures of low-complexity protein segments reveal kinked β sheets that assemble networks Journal Article

Science, 359 (6376), pp. 698–701, 2018.

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Nannenga, Brent L; Gonen, Tamir

MicroED: a versatile cryoEM method for structure determination Journal Article

Emerg Top Life Sci, 2 (1), pp. 1–8, 2018.

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Gallagher-Jones, Marcus; Glynn, Calina; Boyer, David R; Martynowycz, Michael W; Hernandez, Evelyn; Miao, Jennifer; Zee, Chih-Te; Novikova, Irina V; Goldschmidt, Lukasz; McFarlane, Heather T; Helguera, Gustavo F; Evans, James E; Sawaya, Michael R; Cascio, Duilio; Eisenberg, David S; Gonen, Tamir; Rodriguez, Jose A

Sub-ångström cryo-EM structure of a prion protofibril reveals a polar clasp Journal Article

Nat. Struct. Mol. Biol., 25 (2), pp. 131–134, 2018.

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2017

Seidler, P M; Boyer, D R; Rodriguez, J A; Sawaya, M R; Cascio, D; Murray, K; Gonen, T; Eisenberg, D S

Structure-based inhibitors of tau aggregation Journal Article

Nat Chem, 10 (2), pp. 170–176, 2017.

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Vergara, Sandra; Lukes, Dylan A; Martynowycz, Michael W; Santiago, Ulises; Plascencia-Villa, Germán; Weiss, Simon C; de la Cruz, Jason M; Black, David M; Alvarez, Marcos M; López-Lozano, Xochitl; Barnes, Christopher O; Lin, Guowu; Weissker, Hans-Christian; Whetten, Robert L; Gonen, Tamir; Yacaman, Miguel Jose; Calero, Guillermo

MicroED Structure of Au₁₄₆(p-MBA)₅₇ at Subatomic Resolution Reveals a Twinned FCC Cluster Journal Article

J Phys Chem Lett, 8 (22), pp. 5523–5530, 2017.

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Rodriguez, Jose A; Eisenberg, David S; Gonen, Tamir

Taking the measure of MicroED Journal Article

Curr. Opin. Struct. Biol., 46 , pp. 79–86, 2017.

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Wright, Zoë V F; McCarthy, Stephen; Dickman, Rachel; Reyes, Francis E; Sanchez-Martinez, Silvia; Cryar, Adam; Kilford, Iian; Hall, Adrian; Takle, Andrew K; Topf, Maya; Gonen, Tamir; Thalassinos, Konstantinos; Tabor, Alethea B

The Role of Disulfide Bond Replacements in Analogues of the Tarantula Toxin ProTx-II and Their Effects on Inhibition of the Voltage-Gated Sodium Ion Channel Naᵥ1.7 Journal Article

J. Am. Chem. Soc., 139 (37), pp. 13063–13075, 2017.

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Liu, Shian; Hattne, Johan; Reyes, Francis E; Sanchez-Martinez, Silvia; de la Cruz, Jason M; Shi, Dan; Gonen, Tamir

Atomic resolution structure determination by the cryo-EM method MicroED Journal Article

Protein Sci., 26 (1), pp. 8–15, 2017.

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Hughes, Michael P; Sawaya, Michael R; Goldschidt, Lukasz ; Rodriguez Jose A. ad Cascio, Duilio ; Gonen, Tamir ; Eisenberg, David S

Low-complexity domains adhere by reversible amyloid-like interactions between kinked β-sheets Online

bioRxiv 2017.

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de la Cruz, Jason M; Martynowycz, Michael ; Hattne, Johan ; Shi, Dan ; Gonen, Tamir

A method to minimize condenser lens-induced hysteresis effects in a JEOL JEM-3200FSC microscope to enable stable cryoEM low-dose operations Online

bioRxiv 2017.

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Martynowycz, Michael W; Glynn, Calina ; Miao, Jennifer M; de la Cruz, Jason M; Hattne, Johan ; Shi, Dan ; Cascio, Duilio ; Jose, Rodriguez; Gonen, Tamir

MicroED Structures from Micrometer Thick Protein Crystals Online

bioRxiv 2017.

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Krotee, Pascal; Griner, Sarah L; Sawaya, Michael R; Cascio, Duilio; Rodriguez, Jose A; Shi, Dan; Philipp, Stephan; Murray, Kevin; Saelices, Lorena; Lee, Ji; Seidler, Paul; Glabe, Charles G; Jiang, Lin; Gonen, Tamir; Eisenberg, David S

Common fibrillar spines of amyloid-β and human islet amyloid polypeptide revealed by microelectron diffraction and structure-based inhibitors Journal Article

J. Biol. Chem., 293 (8), pp. 2888–2902, 2017.

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de la Cruz, Jason M; Hattne, Johan ; Shi, Dan ; Rodriguez, Jose A; Reyes, Francis E; Gonen, Tamir

Micro- and nanocrystal preparation for MicroED and XFEL serial crystallography by fragmentation of imperfect crystals Online

Nature Protocol Exchange 2017.

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de la Cruz, Jason M; Hattne, Johan; Shi, Dan; Seidler, Paul; Rodriguez, Jose; Reyes, Francis E; Sawaya, Michael R; Cascio, Duilio; Weiss, Simon C; Kim, Sun Kyung; Hinck, Cynthia S; Hinck, Andrew P; Calero, Guillermo; Eisenberg, David; Gonen, Tamir

Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED Journal Article

Nat. Methods, 14 (4), pp. 399–402, 2017.

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Krotee, Pascal; Rodriguez, Jose A; Sawaya, Michael R; Cascio, Duilio; Reyes, Francis E; Shi, Dan; Hattne, Johan; Nannenga, Brent L; Oskarsson, Marie E; Philipp, Stephan; Griner, Sarah; Jiang, Lin; Glabe, Charles G; Westermark, Gunilla T; Gonen, Tamir; Eisenberg, David S

Atomic structures of fibrillar segments of hIAPP suggest tightly mated β-sheets are important for cytotoxicity Journal Article

Elife, 6 , 2017.

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2016

Nannenga, Brent L; Gonen, Tamir

MicroED opens a new era for biological structure determination Journal Article

Curr. Opin. Struct. Biol., 40 , pp. 128–135, 2016.

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Sawaya, Michael R; Rodriguez, Jose; Cascio, Duilio; Collazo, Michael J; Shi, Dan; Reyes, Francis E; Hattne, Johan; Gonen, Tamir; Eisenberg, David S

Ab initio structure determination from prion nanocrystals at atomic resolution by MicroED Journal Article

Proc. Natl. Acad. Sci. U.S.A., 113 (40), pp. 11232–11236, 2016.

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Bale, Jacob B; Gonen, Shane; Liu, Yuxi; Sheffler, William; Ellis, Daniel; Thomas, Chantz; Cascio, Duilio; Yeates, Todd O; Gonen, Tamir; King, Neil P; Baker, David

Accurate design of megadalton-scale two-component icosahedral protein complexes Journal Article

Science, 353 (6297), pp. 389–394, 2016.

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Hsia, Yang; Bale, Jacob B; Gonen, Shane; Shi, Dan; Sheffler, William; Fong, Kimberly K; Nattermann, Una; Xu, Chunfu; Huang, Po‐Ssu.; Ravichandran, Rashmi; Yi, Sue; Davis, Trisha N; Gonen, Tamir; King, Neil P; Baker, David

Design of a hyperstable 60-subunit protein icosahedron Journal Article

Nature, 535 (7610), pp. 136–139, 2016.

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Hattne, Johan; Shi, Dan; de la Cruz, Jason M; Reyes, Francis E; Gonen, Tamir

Modeling truncated pixel values of faint reflections in MicroED images Journal Article

J Appl Crystallogr, 49 (Pt 3), pp. 1029–1034, 2016.

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Shi, Dan; Nannenga, Brent L; de la Cruz, Jason M; Liu, Jinyang; Sawtelle, Steven; Calero, Guillermo; Reyes, Francis E; Hattne, Johan; Gonen, Tamir

The collection of MicroED data for macromolecular crystallography Journal Article

Nat Protoc, 11 (5), pp. 895–904, 2016.

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Meyer, Peter A; Socias, Stephanie; Key, Jason; Ransey, Elizabeth; Tjon, Emily C; Buschiazzo, Alejandro; Lei, Ming; Botka, Chris; Withrow, James; Neau, David; Rajashankar, Kanagalaghatta; Anderson, Karen S; Baxter, Richard H; Blacklow, Stephen C; Boggon, Titus J; Bonvin, Alexandre M J J; Borek, Dominika; Brett, Tom J; Caflisch, Amedeo; Chang, Chung-I; Chazin, Walter J; Corbett, Kevin D; Cosgrove, Michael S; Crosson, Sean; Dhe‐Paganon, Sirano; Cera, Enrico Di; Drennan, Catherine L; Eck, Michael J; Eichman, Brandt F; Fan, Qing R; Ferré‐D’Ámaré, Adrian R; Fromme, Christopher J; Garcia, Christopher K; Gaudet, Rachelle; Gong, Peng; Harrison, Stephen C; Heldwein, Ekaterina E; Jia, Zongchao; Keenan, Robert J; Kruse, Andrew C; Kvansakul, Marc; McLellan, Jason S; Modis, Yorgo; Nam, Yunsun; Otwinowski, Zbyszek; Pai, Emil F; Pereira, Pedro José Barbosa; Petosa, Carlo; Raman, C S; Rapoport, Tom A; Roll‐Mecak, Antonina; Rosen, Michael K; Rudenko, Gabby; Schlessinger, Joseph; Schwartz, Thomas U; Shamoo, Yousif; Sondermann, Holger; Tao, Yizhi J; Tolia, Niraj H; Tsodikov, Oleg V; Westover, Kenneth D; Wu, Hao; Foster, Ian; Fraser, James S; Maia, Felipe R N C; Gonen, Tamir; Kirchhausen, Tom; Diederichs, Kay; Crosas, Mercè; Sliz, Piotr

Data publication with the structural biology data grid supports live analysis Journal Article

Nat Commun, 7 , pp. 10882, 2016.

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Rodriguez, J A; Gonen, T

High-Resolution Macromolecular Structure Determination by MicroED, a cryo-EM Method Book Chapter

Methods in Enzymology, 579 , Chapter 14, pp. 369–392, 2016.

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2015

Rodriguez, Jose A; Ivanova, Magdalena I; Sawaya, Michael R; Cascio, Duilio; Reyes, Francis E; Shi, Dan; Sangwan, Smriti; Guenther, Elizabeth L; Johnson, Lisa M; Zhang, Meng; Jiang, Lin; Arbing, Mark A; Nannenga, Brent L; Hattne, Johan; Whitelegge, Julian; Brewster, Aaron S; Messerschmidt, Marc; Boutet, Sébastien; Sauter, Nicholas K; Gonen, Tamir; Eisenberg, David S

Structure of the toxic core of α-synuclein from invisible crystals Journal Article

Nature, 525 (7570), pp. 486–490, 2015.

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Bale, Jacob B; Park, Rachel U; Liu, Yuxi; Gonen, Shane; Gonen, Tamir; Cascio, Duilio; King, Neil P; Yeates, Todd O; Baker, David

Structure of a designed tetrahedral protein assembly variant engineered to have improved soluble expression Journal Article

Protein Sci., 24 (10), pp. 1695–1701, 2015.

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Hattne, Johan; Reyes, Francis E; Nannenga, Brent L; Shi, Dan; de la Cruz, Jason M; Leslie, Andrew G W; Gonen, Tamir

MicroED data collection and processing Journal Article

Acta Crystallogr A Found Adv, 71 (Pt 4), pp. 353–360, 2015.

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Gonen, Shane; DiMaio, Frank; Gonen, Tamir; Baker, David

Design of ordered two-dimensional arrays mediated by noncovalent protein-protein interfaces Journal Article

Science, 348 (6241), pp. 1365–1368, 2015.

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2014

Huang, Po-Ssu; Oberdorfer, Gustav; Xu, Chunfu; Pei, Xue Y; Nannenga, Brent L; Rogers, Joseph M; DiMaio, Frank; Gonen, Tamir; Luisi, Ben; Baker, David

High thermodynamic stability of parametrically designed helical bundles Journal Article

Science, 346 (6208), pp. 481–485, 2014.

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111 entries « 1 of 3 »