Investigator, Howard Hughes Medical Institute
Professor, Biological Chemistry and Physiology, UCLA

Tamir Gonen is a membrane biophysicist and an expert in crystallography and cryo-EM. Gonen is a professor of Biological Chemistry and Physiology at the David Geffen School of Medicine of the University of California, Los Angeles, an Investigator of the Howard Hughes Medical Institute, and a Member of the Royal Society of New Zealand. He received a Career Development Award from the American Diabetes Association and was an Early Career Scientist of HHMI. Gonen served on several study sections of the National Institutes of Health and acted as ad hoc reviewer for several international funding agencies. In 2011 while leading a lab at the HHMI Janelia Research Campus he began developing microcrystal electron diffraction (MicroED) as a new method for structural biology. In 2017 Dr Gonen moved his laboratory to the David Geffen School of Medicine of the University of California, Los Angeles as an Investigator of the Howard Hughes Medical Institute and a Professor of Biological Chemistry and Physiology, where he continues studying membrane protein structure and function using cryo-EM and MicroED. With this method Dr Gonen has pushed the boundaries of cryo-EM and determined several previously unknown structures at resolutions better than 1 Å. Gonen authored more than 120 publications and several of his past trainees are now faculty around the world at top universities.

Honors

1996Dean’s list—Organic Chemistry, University of Auckland, New Zealand
1996Dean’s list—Inorganic Chemistry, University of Auckland, New Zealand
1997Center for Gene Technology Research Scholarship, University of Auckland, New Zealand
1997Dean’s List—Inorganic Chemistry, University of Auckland, New Zealand
1998Senior prize in Biological Sciences, University of Auckland, New Zealand
1998First class honors in Biological Sciences, University of Auckland, New Zealand
1999University of Auckland Doctoral Scholarship, University of Auckland, New Zealand
2000Contestable Travel Fund Award, University of Auckland, New Zealand
2001Contestable Travel Fund Award, University of Auckland, New Zealand
2009American Diabetes Association Career Development Award
2009Howard Hughes Medical Institute Early Career Scientist
2010New investigator, Science in Medicine Lecture
2012Member, The Royal Society of New Zealand
2017Investigator, Howard Hughes Medical Institute
2018Chair elect, Biophysical Society cryo-EM subgroup
2023Thermo Fisher MicroED Innovation Award
2023A. L. Patterson award, ACA: The Structural Science Society

Publications in PubMed

Publications

194 entries « 1 of 4 »

2024

Unge, Johan; Lin, Jieye; Weaver, Sara J; Her, Ampon Sae; Gonen, Tamir

Compositional Analysis of Complex Mixtures using Automatic MicroED Data Collection

In: Advanced Science, 2024, ISSN: 2198-3844.

Abstract | Links | Altmetric

Xia, Xian; Sung, Po-Yu; Martynowycz, Michael W.; Gonen, Tamir; Roy, Polly; Zhou, Z. Hong

RNA genome packaging and capsid assembly of bluetongue virus visualized in host cells

In: Cell, 2024, ISSN: 0092-8674.

Abstract | Links | Altmetric

Bu, Guanhong; Danelius, Emma; Wieske, Lianne H. E.; Gonen, Tamir

Polymorphic Structure Determination of the Macrocyclic Drug Paritaprevir by MicroED

In: Advanced Biology, vol. 8, iss. 5, pp. 2300570, 2024, ISSN: 2701-0198.

Abstract | Links | Altmetric

Gillman, Cody; Bu, Guanhong; Danelius, Emma; Hattne, Johan; Nannenga, Brent; Gonen, Tamir

Eliminating the Missing Cone Challenge through Innovative Approaches

bioRxiv, 2024.

Abstract | Links | Altmetric

Ruma, Yasmeen N; Bu, Guanhong; Gonen, Tamir

MicroED structure of the C11 cysteine protease Clostripain

bioRxiv, 2024.

Abstract | Links | Altmetric

Lin, Jieye; Bu, Guanhong; Unge, Johan; Gonen, Tamir

Uncovering the Elusive Structures and Mechanisms of Prevalent Antidepressants

bioRxiv, 2024.

Abstract | Links | Altmetric

2023

Lin, Jieye; Unge, Johan; Gonen, Tamir

Unraveling the Structure of Meclizine Dihydrochloride with MicroED

In: Adv Sci (Weinh), pp. e2306435, 2023, ISSN: 2198-3844.

Abstract | Links | Altmetric

Walsh, Martin A; Nannenga, Brent L; Gonen, Tamir; Sexton, Patrick M; Wootten, Denise; Chiu, Wah; Sun, Fei; Carragher, Bridget; Potter, Clinton S; Agard, David; Burley, Stephen K

The next decade in structural biology

In: Structure, vol. 31, no. 11, pp. 1284–1288, 2023, ISSN: 1878-4186.

Abstract | Links | Altmetric

Danelius, Emma; Bu, Guanhong; Wieske, Lianne H E; Gonen, Tamir

MicroED as a Powerful Tool for Structure Determination of Macrocyclic Drug Compounds Directly from Their Powder Formulations

In: ACS Chem Biol, 2023, ISSN: 1554-8937.

Abstract | Links | Altmetric

Hattne, Johan; Clabbers, Max T. B.; Martynowycz, Michael W.; Gonen, Tamir

Electron counting with direct electron detectors in MicroED

In: Structure, 2023, ISSN: 0969-2126.

Abstract | Links | Altmetric

Lin, Jieye; Unge, Johan; Gonen, Tamir

Distinct Conformations of Mirabegron Determined by MicroED

In: Adv Sci (Weinh), pp. e2304476, 2023, ISSN: 2198-3844.

Abstract | Links | Altmetric

Bai, Xiao-Chen; Gonen, Tamir; Gronenborn, Angela M; Perrakis, Anastassis; Thorn, Andrea; Yang, Jianyi

Challenges and opportunities in macromolecular structure determination

In: Nat Rev Mol Cell Biol, 2023, ISSN: 1471-0080.

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Bu, G; Danelius, E; Wieske, L. H; Gonen, T

Polymorphic Structure Determination of the Macrocyclic Drug Paritaprevir by MicroED

bioRxiv, 2023.

Abstract | Links | Altmetric

Lin, Jieye; Unge, Johan; Gonen, Tamir

Unraveling the Structure of Meclizine Dihydrochloride with MicroED

bioRxiv, 2023.

Abstract | Links | Altmetric

Nannenga, Brent L.; Gonen, Tamir

The 2023 Structural Biology Summit at UCLA

In: Structure, vol. 31, pp. 1–2, 2023, ISSN: 0969-2126.

Abstract | Links | Altmetric

Danelius, Emma; Bu, Guanhong; Wieske, Lianne; Gonen, Tamir

MicroED as a powerful tool for structure determination of macrocyclic drug compounds directly from their powder formulations

bioRxiv, 2023.

Abstract | Links | Altmetric

Haymaker, Alison; Bardin, Andrey A.; Gonen, Tamir; Martynowycz, Michael W.; Nannenga, Brent L.

Structure determination of a DNA crystal by MicroED

In: Structure, 2023, ISSN: 0969-2126.

Abstract | Links | Altmetric

Shiriaeva, Anna; Martynowycz, Michael W; Nicolas, William J; Cherezov, Vadim; Gonen, Tamir

MicroED structure of the human vasopressin 1B receptor

bioRxiv, 2023.

Abstract | Links | Altmetric

Gonen, Tamir

Reflections on two decades of MicroED development

In: RefleXions, no. 2, pp. 7–8, 2023.

Links

Gillman, Cody; Nicolas, William J; Martynowycz, Michael W; Gonen, Tamir

Design and implementation of suspended drop crystallization

In: IUCrJ, vol. 10, iss. 4, 2023, ISSN: 2052-2525.

Abstract | Links | Altmetric

Hattne, Johan; Clabbers, Max T. B.; Martynowycz, Michael W.; Gonen, Tamir

Electron-counting in MicroED

bioRxiv, 2023.

Abstract | Links | Altmetric

Lin, Jieye; Unge, Johan; Gonen, Tamir

Distinct Conformations of Mirabegron Determined by MicroED

bioRxiv, 2023.

Abstract | Links | Altmetric

Unge, Johan; Lin, Jieye; Weaver, Sara J; Her, Ampon Sae; Gonen, Tamir

Autonomous MicroED data collection enables compositional analysis

ChemRxiv, 2023.

Abstract | Links | Altmetric

Nguyen, Chi; Lei, Hsiang-Ting; Lai, Louis Tung Faat; Gallenito, Marc J; Mu, Xuelang; Matthies, Doreen; Gonen, Tamir

Lipid flipping in the omega-3 fatty-acid transporter

In: Nat Commun, vol. 14, no. 1, pp. 2571, 2023, ISSN: 2041-1723.

Abstract | Links | Altmetric

Gillman, Cody; Patel, Khushboo; Unge, Johan; Gonen, Tamir

The structure of the neurotoxin palytoxin determined by MicroED

bioRxiv, 2023.

Abstract | Links | Altmetric

Haymaker, Alison; Bardin, Andrey A; Gonen, Tamir; Martynowycz, Michael W; Nannenga, Brent L

Structure determination of a DNA crystal by MicroED

bioRxiv, 2023.

Abstract | Links | Altmetric

Gillman, Cody; Martynowycz, Michael; Nicolas, William Jules; Gonen, Tamir

Design and implementation of suspended drop crystallization

bioRxiv, 2023.

Abstract | Links | Altmetric

Danelius, Emma; Porter, Nicholas J.; Unge, Johan; Arnold, Frances H.; Gonen, Tamir

MicroED Structure of a Protoglobin Reactive Carbene Intermediate

In: J. Am. Chem. Soc., 2023, ISSN: 1520-5126.

Links | Altmetric

Martynowycz, Michael W; Shiriaeva, Anna; Clabbers, Max T B; Nicolas, William J; Weaver, Sara J; Hattne, Johan; Gonen, Tamir

A robust approach for MicroED sample preparation of lipidic cubic phase embedded membrane protein crystals

In: Nat Commun, vol. 14, iss. 1, no. 1, pp. 1086, 2023, ISSN: 2041-1723.

Abstract | Links | Altmetric

Danelius, Emma; Patel, Khushboo; Gonzalez, Brenda; Gonen, Tamir

MicroED in drug discovery

In: Curr Opin Struct Biol, vol. 79, pp. 102549, 2023, ISSN: 1879-033X.

Abstract | Links | Altmetric

2022

Clabbers, Max T. B.; Martynowycz, Michael W.; Hattne, Johan; Gonen, Tamir

Hydrogens and hydrogen-bond networks in macromolecular MicroED data

In: J Struct Biol X, vol. 6, pp. 100078, 2022.

Abstract | Links | Altmetric

Danelius, Emma; Porter, Nicholas J.; Unge, Johan; Arnold, Frances H.; Gonen, Tamir

MicroED structure of a protoglobin reactive carbene intermediate

bioRxiv, 2022.

Abstract | Links | Altmetric

Clabbers, Max T. B.; Martynowycz, Michael W.; Hattne, Johan; Nannenga, Brent L.; Gonen, Tamir

Electron-counting MicroED data with the K2 and K3 direct electron detectors

In: J Struct Biol, vol. 214, iss. 4, 2022.

Abstract | Links | Altmetric

Martynowycz, Michael W.; Shiriaeva, Anna; Clabbers, Max T. B.; Nicolas, William J.; Weaver, Sara J.; Hattne, Johan; Gonen, Tamir

A robust approach for MicroED sample preparation of lipidic cubic phase embedded membrane protein crystals

bioRxiv 2022, visited: 26.07.2022.

Abstract | Links | Altmetric

Clabbers, Max T. B.; Martynowycz, Michael W.; Hattne, Johan; Nannenga, Brent L.; Gonen, Tamir

Electron-counting MicroED data with the K2 and K3 direct electron detectors

bioRxiv, 2022.

Abstract | Links | Altmetric

Nguyen, Chi; Lei, Hsiang-Ting; Lai, Louis Tung Faat; Gallenito, Marc J.; Matthies, Doreen; Gonen, Tamir

Lipid flipping in the omega-3 fatty-acid transporter

bioRxiv 2022, visited: 01.06.2022.

Abstract | Links | Altmetric

Martynowycz, Michael W.; Gonen, Tamir

Unlocking the potential of microcrystal electron diffraction

In: Physics Today, vol. 75, iss. 6, pp. 38–42, 2022.

Abstract | Links | Altmetric

Martynowycz, Michael W; Clabbers, Max T B; Hattne, Johan; Gonen, Tamir

Ab initio phasing macromolecular structures using electron-counted MicroED data

In: Nat Methods, vol. 19, iss. 6, pp. 724–729, 2022, ISSN: 1548-7105.

Abstract | Links | Altmetric

Porter, Nicholas; Danelius, Emma; Gonen, Tamir; Arnold, Frances

Biocatalytic Carbene Transfer Using Diazirines

ChemRxiv, 2022.

Abstract | Links | Altmetric

Porter, Nicholas J; Danelius, Emma; Gonen, Tamir; Arnold, Frances H

Biocatalytic Carbene Transfer Using Diazirines

In: J Am Chem Soc, 2022, ISSN: 1520-5126.

Abstract | Links | Altmetric

Clabbers, Max T. B.; Martynowycz, Michael W.; Hattne, Johan; Gonen, Tamir

Hydrogens and hydrogen-bond networks in macromolecular MicroED data

bioRxiv, 2022.

Abstract | Links | Altmetric

Clabbers, Max T B; Shiriaeva, Anna; Gonen, Tamir

MicroED: conception, practice and future opportunities

In: IUCrJ, vol. 9, no. Pt 2, pp. 169–179, 2022, ISSN: 2052-2525.

Abstract | Links | Altmetric

Gallenito, Marc J; Gonen, Tamir

Studying membrane proteins with MicroED

In: Biochem Soc Trans, vol. 50, no. 1, pp. 231–239, 2022, ISSN: 1470-8752.

Abstract | Links | Altmetric

2021

Gonen, Tamir; Nannenga, Brent (Ed.)

cryoEM: Methods and Protocols

Springer US, 2021, ISBN: 978-1-0716-0965-1.

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Martynowycz, Michael W; Clabbers, Max T B; Unge, Johan; Hattne, Johan; Gonen, Tamir

Benchmarking the ideal sample thickness in cryo-EM

In: Proc Natl Acad Sci U S A, vol. 118, no. 49, 2021, ISSN: 1091-6490.

Abstract | Links | Altmetric

Clark, Lisa J.; Bu, Guanhong; Nannenga, Brent L.; Gonen, Tamir

MicroED for the study of protein–ligand interactions and the potential for drug discovery

In: Nat Rev Chem, vol. 5, pp. 853–858, 2021.

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Martynowycz, Michael W.; Clabbers, Max T. B.; Hattne, Johan; Gonen, Tamir

Ab initio phasing macromolecular structures using electron-counted MicroED data

bioRxiv, 2021.

Abstract | Links | Altmetric

Martynowycz, Michael W; Gonen, Tamir

Protocol for the use of focused ion-beam milling to prepare crystalline lamellae for microcrystal electron diffraction (MicroED)

In: STAR Protoc, vol. 2, no. 3, pp. 100686, 2021, ISSN: 2666-1667.

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Martynowycz, Michael W.; Shiriaeva, Anna; Ge, Xuanrui; Hattne, Johan; Nannenga, Brent L.; Cherezov, Vadim; Gonen, Tamir

MicroED structure of the human adenosine receptor determined from a single nanocrystal in LCP

In: Proc Natl Acad Sci U S A, vol. 118, no. 36, pp. e2106041118, 2021.

Abstract | Links | Altmetric

Martynowycz, Michael W.; Clabbers, Max T. B.; Unge, Johan; Hattne, Johan; Gonen, Tamir

Benchmarking ideal sample thickness in cryo-EM using MicroED

bioRxiv, 2021.

Abstract | Links | Altmetric

194 entries « 1 of 4 »