CV for Tamir Gonen, PhD, MRSNZ

@article{gonen_2000,

title = {Galectin-3 Is Associated with the Plasma Membrane of Lens Fiber Cells},

author = {Tamir Gonen and Paul Donaldson and Joerg Kistler},

url = {https://cryoem.ucla.edu/wp-content/uploads/gonen_2000.pdf, Main text},

year  = {2000},

date = {2000-01-01},

journal = {Investigative Ophthalmology and Visual Science},

volume = {41},

number = {1},

pages = {199--203},

abstract = {PURPOSE: To discover proteins that have the potential to contribute to the tight packing of fiber cells in the lens.

METHODS: Crude  fiber  cell  membranes  were  isolated  from  ovine  lens  cortex.  Proteins  were separated by two-dimensional gel electrophoresis, and selected protein spots identified by micro-sequencing.  The  identification  of  galectin-3  was  confirmed  by  immunoblotting  with  a  specific antibody. The association of galectin-3 with the fiber cell plasma membrane was investigated using immunofluorescence microscopy, solubilization trials with selected reagents, and immunoprecipitation to identify candidate ligands.

RESULTS: A  cluster  of  three  protein  spots  with  an  apparent  molecular  weight  of  31,000  and isoelectric points ranging between 7 and 8.5 were resolved and identified as galectin-3. This protein was associated peripherally with the fiber cell plasma membrane and interacted with MP20, an abundant  intrinsic  membrane  protein  that  had  been  identified  previously  as  a  component  of membrane junctions between fiber cells.

CONCLUSIONS: The detection of galectin-3 in the lens is a novel result and adds to the growing list of lens proteins with adhesive properties. Its location at the fiber cell membrane and its association with the junction-forming MP20 is consistent with a potential role in the development or maintenance of the tightly packed lens tissue architecture. (Invest Ophthalmol Vis Sci. 2000;41:199 –203)},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{kistler_1999,

title = {Molecular Solutions To Tissue Transparency},

author = {Joerg Kistler and Rachelle Merriman-Smith and Miriam Young and Tamir Gonen and Dougal Cowan and Kaa-Sandra Chee and Jun Sheng Lin and Colin Green and Lorenz Hasler and Andreas Engel and Paul Donaldson},

url = {https://cryoem.ucla.edu/wp-content/uploads/kistler_1999.pdf, Main text},

year  = {1999},

date = {1999-08-01},

journal = {N.Z. Biosciences},

pages = {35--37},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{baker_1999,

title = {More Than Just A Pretty Picture},

author = {Ted Baker and Peter Metcalf and Clyde Smith and Vic Arcus and Rachael Ashton and Heather Baker and Mark Banfield and Jenny Cross and David Drew and David Goldstone and Tamir Gonen and Peter Haebel and Caroline Holliss and Ivan Ivanovich and Todd Kagawa and Richard Kidd and Nayden Koon and Sabine Leydier and Shaun Lott and Andrew McCarthy and Didier Nurizzo and Steve Shewry and Jill Sigrell and Xiaolin Sun},

url = {https://cryoem.ucla.edu/wp-content/uploads/baker_1999.pdf, Main text},

year  = {1999},

date = {1999-08-01},

journal = {N.Z. Biosciences},

pages = {32--35},

keywords = {},

pubstate = {published},

tppubtype = {article}

}