Tamir Gonen

CV for Tamir Gonen, Ph.D., MRSNZ

    Investigator, Howard Hughes Medical Institute at UCLA

2005 – 2011                Assistant Professor of Biochemistry, University of Washington, Seattle

2009 – 2011                Howard Hughes Medical Institute Early Career Scientist

2011                            Associate Professor of Biochemistry (Tenured), University of Washington

2011 – present           Affiliate Professor of Biochemistry, University of Washington, Seattle, WA

2011 – 2017                Group Leader, HHMI, Janelia Research Campus, Ashburn VA

2017 – Present           Investigator, Howard Hughes Medical Institute.

2017 – present           Professor of Biological Chemistry and Physiology, David Geffen Medical School. University of Califormnia, Los Angeles CA.

        Other links:   Howard Hughes Medical Institute - Gonen Profile
                             Janelia Research Campus - Gonen Lab




1996                 Deans List - Organic Chemistry, University of Auckland, New Zealand
1996                 Deans List - Inorganic Chemistry, University of Auckland, New Zealand
1997                 Center for Gene Technology Research Scholarship, University of Auckland, New Zealand
1997                 Deans List - Inorganic Chemistry, University of Auckland, New Zealand
1998                 Senior Prize in Biological Sciences, University of Auckland, New Zealand
1998                 First Class Honors in Biological Sciences, University of Auckland, New Zealand
1999                 University of Auckland Doctoral Scholarship, University of Auckland, New Zealand
2000                 Contestable Travel Fund Award, University of Auckland, New Zealand
2001                 Contestable Travel Fund Award, University of Auckland, New Zealand
2009                 American Diabetes Association Career Development Award
2009                 Howard Hughes Medical Institute Early Career Scientist
2010                 New Investigator Science in Medicine Lecture
2012                 Member, The Royal Society of New Zealand
2017                 Investigator, Howard Hughes Medical Institute
2018                 Chair elect, Biophysical Society cryoEM subgroup
Publications in Pubmed



Liu Y., Gonen S., Gonen T. and Yeates TO. Near-Atomic Cryo-EM Imaging of a Small Protein Displayed on a Designed Scaffolding System. BioRxiv: https://doi.org/10.1101/212233 (2017).

Seidler PM., Boyer DR., Rodriguez JA., Sawaya MR., Cascio D., Murray K., Gonen T. and Eisenberg DS. Structure-based inhibitors of tau aggregation. Nature Chemistry - In Press. (2017).

Wright ZVF., McCarthy S., Dickman R., Reyes FE., Sanchez-Martinez S., Cryar A., Kilford I, Hall A., Takle AK., Topf M., Gonen T, Thalassinos K., Tabor AB. The role of disulfide bond replacements in analogues of the tarantula toxin ProTx-II and their effects on inhibition of the voltage-gated sodium ion channel Nav1.7. Journal of the American Chemical Society 139 (37): 13063 - 13075 (2017).

Guenther EL, Ge P, Trihn H, Sawaya MR, Cascio D, Boyer DR, Gonen T, Zhou ZH, Eisenberg DS. Prodigious Packing Polymorphism of a Segment of Amyloid-forming Protein TDP-43 Revealed by Molecular Structures. Nature Structural and Molecular Biology – In Press (2017).

Vergara S., Lukes D., Santiago U., Plascencia-Villa G., Weiss S., Martynowycz MW., Cruz MJ., Black DM., Alvarez MM., Lopez-Lozano X., Weissker HC., Whetten RL*., Gonen T*. Yacaman JM*. and Calero G*. MicroED structure of Au146(p-MBA)57 at subatomic resolution reveals a twinned FCC cluster. The Journal of Physical Chemistry Letters 8: 5523 - 5530 (2017).

Hughes MP., Sawaya MR., Goldschmidt L., Rodriguez JA., Cascio D., Gonen T and Eisenberg DS. Low-complexity domains adhere by reversible amyloid-like interactions between kinked β-sheets. BioRxiv https://doi.org/10.1101/153817 (2017).

Cruz MJ., Martynowycz MW., Hattne J., Shi D. and Gonen T*. A method to minimize condenser lens-induced hysteresis effects in a JEOL JEM-3200FSC microscope to enable stable cryoEM low-dose operations. BioRxiv https://doi.org/10.1101/153395 (2017).

Martynowycz MW., Glynn C., Miao J., de la Cruz MJ., Hattne J., Shi D., Cascio D., Rodriguez J. and Gonen T*. MicroED Structures from Micrometer Thick Protein Crystals. BioRxiv https://doi.org/10.1101/152504 (2017).

Rodriguez JA*, Eisenberg D and Gonen T*. Taking the measure of MicroED. Current Opinion in Structural Biology 46: 79 - 86 (2017).

Cruz MJ., Hattne J., Shi D., Seidler P., Rodriguez JA., Reyes FE., Sawaya MR., Duilio C., Weiss SC., Kim SK., Hinck CS., Hinck AP., Calero, G., Eisenberg D. and Gonen T* Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED. Nature Methods 14 (4) : 399 - 402 (2017).

Cruz MJ., Hattne J., Shi D., Rodriguez JA., Reyes FE. and Gonen T* Micro- and nanocrystal preparation for MicroED and XFEL serial crystallography by fragmentation of imperfect crystals. Nature Protocol Exchange DOI 10.1038/protex.2017.010: (2017).

Krotee P., Rodriguez JA., Sawaya MR., Cascio D., Reyes FE., Shi D., Hattne J., Nannenga B., Oskarrson M., Griner S., Jiang L., Westermark G., Gonen T. and Eisenberg D. Atomic Structures of Fibrillar Segments of Human Islet Amyloid Polypeptide Suggest Tightly Mated β-Sheets Drive Cytotoxicity. eLife 6:e19723: 1 - 26 (2017).


Nannenga BL. and Gonen T. MicroED opens a new era for biological structure determination. Current Opinion in Structural Biology 40: 128 - 135 (2016).

Sawaya MR., Rodriguez J., Cascio D., Collazo M., Shi D., Reyes FE., Hattne J., Gonen T* and Eisenberg DS* Ab Initio structure determination from prion nanocrystals at atomic resolution by MicroED. Proceedings of the National Academy of Sciences 40 (113): 11232 - 11236 (2016).

Liu S., Hattne J., Refes EF., Sanchez-Martinez S., de la Cruz MJ., Shi D and and Gonen T*. Atomic resolution structure determination by the cryo-EM method MicroED. Protein Science 26: 8 - 15 (2016).

Bale JB., Gonen S., Liu Y., Sheffler W., Ellis D., Thomas C, Cascio D., Yeates TO., Gonen T., King NP. and Baker D. Accurate design of megadalton-scale multi-component icosahedral protein complexes. Science 353 (6297): 389 - 394 (2016).

Rodriguez J.A. and Gonen T*. High-resolution macromolecular structure determination by MicroED, a cryo-EM method. Methods in Enzymology 579: 369 - 392 (2016).

Hsia Y., Bale JB., Gonen S., Shi D., Sheffler W., Fong KK., Nattermann U., Xu C., Huang P., Ravichandran R., Yi S., Davis TN., Gonen T., King NP., and Baker D. Design of a hyperstable 60-subunit protein icosahedron. Nature 535 (7610): 136 - 139. (2016).

Hattne J., Shi D., de la Cruz J., Reyes FE., and Gonen T*. Modeling truncated intensities of faint reflections in MicroED images. Journal of Applied Crystallography 49: 1029 - 1034 (2016).

Shi D., Nannenga B., de la Cruz J., Jiu L., Guillermo C., Hattne J., Reyes FE., Sawtelle S. and Gonen T*. The collection of MicroED data for macromolecular crystallography. Nature Protocols 11 (5) : 895 - 904 (2016).

Meyer PA, Socias S, Key J, Ransey E, Tjon EC, Buschiazzo A, Lei M, Botka C, Withrow J, Neau D, Rajashankar K, Anderson KS, Baxter RH, Blacklow SC, Boggon TJ, Bonvin AM, Borek D, Brett TJ, Caflisch A, Chang CI, Chazin WJ, Corbett KD, Cosgrove MS, Crosson S, Dhe-Paganon S, Di Cera E, Drennan CL, Eck MJ, Eichman BF, Fan QR, Ferré-D'Amaré AR, Christopher Fromme J, Garcia KC, Gaudet R, Gong P, Harrison SC, Heldwein EE, Jia Z, Keenan RJ, Kruse AC, Kvansakul M, McLellan JS, Modis Y, Nam Y, Otwinowski Z, Pai EF, Pereira PJ, Petosa C, Raman CS, Rapoport TA, Roll-Mecak A, Rosen MK, Rudenko G, Schlessinger J, Schwartz TU, Shamoo Y, Sondermann H, Tao YJ, Tolia NH, Tsodikov OV, Westover KD, Wu H, Foster I, Fraser JS, Maia FR, Gonen T, Kirchhausen T, Diederichs K, Crosas M, Sliz P. Data publication with the Structural biology data grid supports live analysis. Nature Communications 7 (10882): 1 - 12 (2016).


Rodriguez A.J., Ivanova M., Sawaya MR., Cascio D., Reyes F., Shi D., Sangwan S., Guenther EL., Johnson L, Zhang M., Jiang L., Arbing M., Nannega B., Hattne J., Whitelegge J., Brewster AS., Messerschmidt M., Boutet S., Sauter NK., Gonen T* and Eisenberg D* Structure of the toxic core of a-synuclein from invisible crystals. Nature 525 (7570): 486 - 490 (2015).

Bale JB., Park RU., Liu Y., Gonen S., Gonen T., Cascio D., King NP., Yeates TO., and Baker D. Structure of a designed tetrahedral protein assembly variant engineered to have improved soluble expression. Protein Science 24 (10): 1695 - 1701 (2015).

Hattne J., Reyes FE., Nannenga BL., Shi D., de la Cruz J., Leslie AGW. And Gonen T*. MicroED data collection and processing. Acta Crystallographica section A. A71: 353 - 360 (2015).

Gonen S., DiMIao F., Gonen T* and Baker D*. Design of ordered two-dimensional arrays mediated by noncovalent protein-protein interfaces. Science 348 (6241): 1365 - 1368 (2015).


Nannenga BL., Shi D., Hattne J., Reyes F. and Gonen T*. Structure of catalase determined by MicroED. eLife 3:e03600: 1 - 11. (2014).

Huang PS, Oberdorfer G., Xu C., Pei XY., Nannenga BL., Rogers JM., DiMIao F., Gonen T., Luisi B. and Baker D. High thermodynamic stability of parametrically designed helical bundles. Science 346: 481 - 485 (2014).

Nannenga BL, Shi D., Leslie AGW. and Gonen T*. High-resolution structure determination by continuous rotation data collection in MicroED. Nature Methods 11 (9): 927 - 930 (2014).

Wisedchaisri G., Park MS., Iadanza MG., Zheng H. and Gonen T*. Proton-coupled sugar transport in the prototypical major facilitator superfamily protein XylE. Nature Communications (5) 4521: 1 - 11 (2014).

Gonen T* and Waksman G*. Recent methods in the study of membrane protein structure. Current Opinion in Structural Biology 27: 1 - 2 (2014).

Russell AB., Wexler AG., Harding BN., Whitney JC., Bohn AJ., Goo YA., Barry NA., Zheng H., Peterson SB., Gonen T., Goodlett DR., Goodmann AL. and Mougous JD Discovery and characterization of a type VI secretion related interbacterial antagonism pathway in the Phylum Bacteroidetes. Cell Host & Microbe 16: 227 - 236 (2014).

King NP, Bale J, Sheffler W, McNamara DE., Gonen S., Gonen T., Yeates TO. and Baker D. Accurate design of coassembling multi-component protein nanomaterials. Nature 510 (7503): 103 - 108 (2014).

Iadanza MG. and Gonen T*. A suite of software for processing MicroED data of extremely small protein crystals. Journal of Applied Crystallography. 47: 1140 - 1145 (2014).

Nannenga BL. and Gonen T*. Protein structure determination by MicroED. Current Opinion in Structural Biology. 27: 24 - 31 (2014).

Anderson TM., Clay MC., Cioffi AG., Diaz KA., Hisao G.S., Nieuwkoop AJ., Comellas G., Wang S., Uno BE., Maryum N., Gonen T., Rienstra CM. and Burke MD. Amphotericin kills yeast by forming a sterol sponge. Nature Chemical Biology. 10 (5): 400 - 406 (2014).


Shi D., Nannenga B., Iadanza GM. and Gonen T*. Three Dimensional electron crystallography of protein microcrystals. eLife. 2:e01345: 1 - 17 (2013).

Smith FD., Reichow LS., Esseltine JL., Shi D., Langeberg LK., Scott J* and Gonen T*. Intrinsic disorder within an AKAP-Protein kinase A complex guides local substrate phosphorylation. eLife. *Co-Corresponding authors 2:e01319: 1 - 19 (2013).

Zheng H., Wisedchaisri G. and Gonen T*. Crystal structure of a nitrate/nitrite exchanger. Nature 497: 647 - 651 (2013).

Reichow SL, Clemens DM., Freites JA., Németh-Cahalan KL., Heyden M., Tobias DJ., Hall JE*. and Gonen T* Allosteric mechanism of water channel gating by Ca2+-calmodulin. Nature Structural and Molecular Biology. *Co-Corresponding authors 20 (9): 1085 - 1092 (2013).

Reichow SL. and Gonen T*. The dynamic modulation of water permeability in the lens aquaporin-0. Microscopy and Microanalysis 19 (S2): 50 - 51 (2013).

Gold MG, Gonen T. and Scott JD. Local cAMP signaling in disease at a glance. Journal of Cell Science 126: 4537 - 4543 (2013).

Silverman J., Zheng H., Gonen T. and Mougous J. Haemolysin Co-regulated Protein is an Exported Receptor and Chaperone of Type VI Secretion Substrates. Molecular Cell. 51: 584 - 593 (2013).

Nannenga LB., Iadanza MG., Vollmar BS and Gonen T*. Electron crystallography of membrane proteins: crystallization and screening strategies using negative stain electron microscopy. Current Protocols in Protein Science. 17 (15): 1 - 11 (2013).

Filbin ME., Vollmar BS., Shi D., Gonen T*. and Kieft JS*. HCV IRES manipulates the ribosome to promote the switch from translation initiation to elongation. Nature Structural and Molecular Biology. *Co-Corresponding authors. 20 (2): 150 - 158 (2013).

Shi L., Zheng H., Zheng H., Borkowski B., Shi D., Gonen T and Jiang QS. Voltage sensor ring in a native structure of a membrane-embedded potassium channel. Proceedings of the National Academy of Sciences - Retracted. 110 (9): 3369 - 3374 (2013).

Stoke DL., Ubarretxena I., Gonen T. and Engel A. High throughput methods for electron crystallography. Methods in Molecular Biology. 955: 273 - 296 (2013).

Gonen T*. The collection of high-resolution electron diffraction data. Methods in Molecular Biology. 955: 153 - 169 (2013).

Wisedchaisri G. and Gonen T*. Phasing electron diffraction data by molecular replacement: strategy for structure determination and refinement. Methods in Molecular Biology. 955: 243 - 272 (2013).


Gonen S., Akiyoshi B., Iadanza MG., Shi D., Duggan N., Biggins S*. and Gonen T*. The structure of purified kinetochores reveals multiple microtubule-attachment sites. Nature Structural and Molecular Biology. *Co-Corresponding authors. 19 (9): 925 - 930 (2012).

Umbreit NT., Gestaut DR., Tien JF., Vollmar BS., Gonen T, Asbury CL and Davis TN. The Ndc80 kinetochore complex directly modulates microtubule dynamics. Proceedings of the National Academy of Sciences 109 (40): 16113 - 16118 (2012).

King NP., Sheffler W., Sawaya MR., Vollmar BS., Sumida JP., Andre I., Gonen T., Yeates TO. and Baker D. Computational design of self-assembling protein nanomaterials with atomic level accuracy. Science 336: 1171 - 1174 (2012).

Jiang Q*. and Gonen T*. The influence of lipids on voltage gated ion channels. Current Opinion in Structural Biology. *Co-Corresponding authors. 22: 529 - 536 (2012).

Gonen T*. and Waksman G*. Recent progress in membrane protein structures and investigation methods. Current Opinion in Structural Biology. *Co-Corresponding authors. 22: 467 - 468 (2012).

Gold MG., Reichow SL., O’Neill SE., Weisbrod CR., Langeberg LK., Bruce JE., Gonen T*. and Scott JD*. AKAP2 anchors PKA with aquaporin-0 to support ocular lens transparency. EMBO Molecular Medicine. *Co-Corresponding authors. 4: 15-26 (2012).

Lo M., Brett CL., Plemel R., Vignali M., Fields S., Gonen T. and Merz A. Intrinsic tethering activity of endosomal Rab proteins. Nature Structural & Molecular Biology 19: 40 - 47 (2012).


Wisedchaisri W., Reichow SL. and Gonen T*. Advances in structural and functional analysis of membrane proteins by electron crystallography. Structure 19: 1381 - 1393 (2011).

Wisedchaisri G. and Gonen T*. Fragment-based phase extension for three-dimensional structure determination of membrane proteins by electron crystallography. Structure 19: 976 - 987 (2011).

Korotkov KV., Gonen T and Hol WGJ. Secretins: dynamic channels for protein transport across membranes. Trends in Biochemical Sciences 36 (8) : 433 - 443 (2011).

Budzinski KL., Sgro AE., Fujimoto BS., Gadd JC., Shuart NG., Gonen T., Bajjalieh SM and Chiu DT. Synaptosomes as a platform for loading nanoparticles into synaptic vesicles. ACS Chem Neuroscience 18: 236 - 241 (2011).

Reichow SL., Korotkov KV., Gonen M., Sun J., Delarosa J., Hol WGJ* and Gonen T*. The binding of cholera toxin to the periplasmic vestibule of the type II secretion channel. Channels. *Co-Corresponding authors. 5(3): 215 - 218 (2011).

Jehle S., Vollmar B., Bardiaux B., Dove K., Rajagopal P., Gonen T*., Oschkinat H* and Klevit RE*. The N-terminal domain of αB-crystallin provides a conformational switch for multimerization and structural heterogeneity. Proceedings of the National Academy of Sciences. *Co-Corresponding authors. 108: 6409 - 6414 (2011).


Akiyoshi B., Sarangapani KK., Powers AF., Nelson CR., Reichow SL., Arellano-Santoyo H., Gonen T., Ranish JA, Asbury CL. and Biggins S. Tension directly stabilizes reconstituted kinetochore-microtubule attachments. Nature 468: 576 - 581 (2010).

Reichow SL., Korotkov KV., Hol WGJ*. and Gonen T*. Structure of the cholera toxin secretion channel in its closed state. Nature Structural & Molecular Biology. *Co-Corresponding authors. 17: 1226 - 1232 (2010).

Wisedchaisri G., Dranow DM., Lie TJ., Bonanno JB., Patskovsky Y., Ozyurt SA., Sauder JM., Almo SC., Wasserman SR., Burley SK., Leigh JA. and Gonen T*. Structural underpinnings of nitrogen regulation by the prototypical nitrogen-responsive transcriptional factor NrpR. Structure 18: 1512 - 1521 (2010).

Hall-Sedlak R., Hnilova M., Gachelet E., Przybyla L., Dranow DM., Gonen T., Sarikaya M., Tamerler C. and Traxler B. An engineered DNA-binding protein self-assembles Metallic Nanostructures. Chem BioChem 11 (15): 2108-2112 (2010).

Sanowar S., Singh P., Pfuetzner RA., Andre I., Zheng H., Spreter T., Strynadka NCJ., Gonen T., Baker D., Goodlett DR. and Miller SI. Interactions of the transmembrane polymeric rings of the Salmolella typhimurium type III secretion system. mBIO 1 (3): e00158-10 (2010).

Tien JF., Umbreit NT., Gestaut DR., Frank AD., Cooper J., Wordeman L., Gonen T., Asbury CL. and Davis TN. Cooperation of the Dam1 and Ndc80 kinetochore complexes enhances microtubule coupling and is regulated by Aurora B. Journal of Cell Biology 189: 713 - 723 (2010).

Zheng H., Taraska J., Merz A. and Gonen T*. The prototypical H+/galactose symporter GalP assembles into functional trimers. Journal of Molecular Biology 396: 593 - 601 (2010).


Reichow L.S. and Gonen T*. Lipid-protein interactions probed by electron crystallography. Current Opinion in Structural Biology 19: 560 - 565 (2009).


Zheng H., Wisedchaisri G. and Gonen T*. Single particle electron cryomicroscopy of bacteriophage P22 portal protein complexes. Microscopy and Microanalysis 14 (S2): 1572 - 1573 (2008).

Engel A., Fujiyoshi Y., Gonen T. and Walz T. Junction-forming aquaporins. Current Opinion in Structural Biology 18: 229-235 (2008).

Gonen T., Hite R.K., Cheng Y., Petre B.M., Kistler J. and Walz T. Polymorphic assemblies and crystalline arrays of lens tetraspanin MP20. Journal of Molecular Biology 376: 380-392 (2008).

Andrews, S.A., Reichow, L.S. and Gonen T*. Electron crystallography of aquaporins. IUBMB Life 60: 430 - 436 (2008).

Reichow L.S. and Gonen T*. Non-canonical binding of calmodulin to aquaporin-0: implications for channel regulation. Structure 16: 1389 - 1398 (2008).

Zheng H., Olia A.S., Gonen M., Andrews S., Cingolani G*. and Gonen T*. A conformational switch in bacteriophage P22 portal protein primes genome injection. Molecular Cell. *Co-Corresponding authors. 29: 376 - 383 (2008).


Viadiu H., Gonen T. and Walz T. Projection map of aquaporin-9 at 7 A resolution. Journal of Molecular Biology 367: 80-88 (2007).

Hite R.K., Gonen T., Harrison S.C. and Walz T. Interactions of lipids with aquaporin-0 and other membrane proteins. Pflugers Archive - European Journal of Physiology 456: 651-661 (2007).

Franck A.D., Powers A.F., Gestaut D.R., Gonen T., Davis T.N. and Asbury C.L. Tension applied through the Dam1 complex promotes microtubule elongation providing a direct mechanism for length control in mitosis. Nature Cell Biology 9(7): 832-837 (2007).


Gonen T*. and Walz T*. The structure of aquaporins. Quarterly Reviews of Biophysics. *Co-Corresponding authors. 39, 4: 361-396 (2006).


Gonen T., Cheng Y., Sliz P., Hiroaki Y., Fujiyoshi Y., Harrison SC. and Walz T. Lipid–protein interactions in double layered two dimensional crystals of AQP0. Nature 438: 633-638 (2005).


Gonen T., Cheng Y., Kistler J. and Walz T. Aquaporin-0 membrane junctions form upon proteolytic cleavage. Journal of Molecular Biology 342(4): 1337-45 (2004).

Gonen T., Sliz P., Cheng Y., Kistler J. and Walz T. Aquaporin-0 membrane junctions reveal the structure of a closed water pore. Nature 429 (6988): 193-7 (2004).


Grey AC., Jacobs MD., Gonen T., Kistler J. and Donaldson PJ. Insertion of MP20 into lens fibre cell plasma membranes correlates with the formation of an extracellular diffusion barrier. Experimental Eye Research 77(5): 567-74 (2003).


Gonen T., Grey AC., Jacobs MD., Donaldson PJ. and Kistler J. MP20, the second most abundant lens membrane protein and member of the tetraspanin superfamily, joins the list of ligands of galectin-3. BioMed Central - Cell Biology 2: 17 (2001).


Gonen T., Donaldson PJ. and Kistler J. Galectin-3 – a new adhesion molecule in the lens. Investigative Ophthalmology and Visual Science 41: 199-203 (2000).


Baker EN., Metcalf P., Smith C., Arcus V., Ashton R., Baker H., Banfield M., Cross J., Drew D., Goldstone D., Gonen T., Haebel P. et al. More than a pretty picture. N.Z. Biosciences August: 32-35 (1999).

Kistler J., Merriman-Smith R., Young M., Gonen T., Cowan D. et al. Molecular solutions to tissue transparency. N.Z. Biosciences August: 35-37 (1999).